Motta M
Peptides. 1985;6 Suppl 3:131-5. doi: 10.1016/0196-9781(85)90363-8.
The effect of dermorphin and sauvagine, two new peptides originally isolated from amphibian skin, on the release of gonadotropins and prolactin was studied "in vivo" and "in vitro." The intraventricular administration of dermorphin to castrated male rats induces a significant decrease in serum LH, but not FSH, levels. Subcutaneous, as well as intraventricular, injections of dermorphin to normal male rats induce a statistically significant increase in serum prolactin levels, which is counteracted by naloxone. In the isolated and dispersed rat pituitary cell preparation, the addition of dermorphin to the media does not induce any alteration in prolactin output. Intraventricular administrations of sauvagine are ineffective in influencing the secretion of both LH and FSH. This peptide is also unable to modify it when tested "in vitro". On the contrary, subcutaneous injections of sauvagine to either normal male or lactating rats decrease serum prolactin levels. This prolactin inhibitory effect is also present "in vitro." These results suggest that dermorphin and sauvagine modulate the secretory activity of the anterior pituitary gland. However, the mechanism of control is different: dermorphin needs the mediation of brain structures, while sauvagine acts directly at the pituitary level.
对最初从两栖动物皮肤中分离出的两种新肽——皮啡肽和蛙皮素，进行了“体内”和“体外”研究，以观察它们对促性腺激素和催乳素释放的影响。给去势雄性大鼠脑室内注射皮啡肽可导致血清促黄体生成素（LH）水平显著降低，但促卵泡生成素（FSH）水平未受影响。给正常雄性大鼠皮下或脑室内注射皮啡肽，可使血清催乳素水平出现具有统计学意义的升高，而纳洛酮可抵消这种升高。在分离和分散的大鼠垂体细胞制剂中，向培养基中添加皮啡肽不会引起催乳素分泌的任何改变。脑室内注射蛙皮素对LH和FSH的分泌均无影响。在“体外”试验时，这种肽也无法对其产生影响。相反，给正常雄性大鼠或泌乳大鼠皮下注射蛙皮素可降低血清催乳素水平。这种催乳素抑制作用在“体外”也存在。这些结果表明，皮啡肽和蛙皮素可调节垂体前叶的分泌活动。然而，控制机制有所不同：皮啡肽需要脑结构的介导，而蛙皮素则直接作用于垂体水平。
