CRISPR-Cas12a 检测 DNA 糖基化酶 DNA 修饰转换。

CRISPR-Cas12a detection of DNA glycosylases DNA modification switching.

机构信息

Guangdong Provincial Key Laboratory of Digestive Cancer Research, Digestive Diseases Center, Scientific Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, 518107, P. R. China.

School of Medicine, Sun Yat-sen University, Shenzhen, Guangdong, 518107, P. R. China.

出版信息

Chem Commun (Camb). 2024 Oct 24;60(86):12569-12572. doi: 10.1039/d4cc04180a.

DOI:10.1039/d4cc04180a

PMID:39385597

Abstract

A programmable CRISPR-Cas12a system for selective detection of various DNA glycosylases is described. By temporarily inactivating Cas12a through the introduction of specific DNA modifications in the complementary DNA strand of Cas12a's crRNA, the system is able to detect the target DNA glycosylases. This approach addresses critical gaps in current CRISPR-Cas12a diagnostics for non-nucleic acid detection beyond the limitations of aptamers.

摘要

描述了一种可编程的 CRISPR-Cas12a 系统，用于选择性检测各种 DNA 糖基化酶。通过在 Cas12a 的 crRNA 的互补 DNA 链中引入特定的 DNA 修饰，暂时使 Cas12a 失活，该系统能够检测到目标 DNA 糖基化酶。这种方法解决了当前 CRISPR-Cas12a 诊断在非核酸检测方面的关键空白，超越了适体的限制。