State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences - Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430071, China.
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Chem Commun (Camb). 2020 Oct 20;56(83):12526-12529. doi: 10.1039/d0cc05540a.
We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.
我们首次报道，激活的 CRISPR-Cas12a 系统可切割 DNA 四链体（G4）。通过荧光共振能量转移（FRET）、圆二色性（CD）、凝胶电泳和 NMR 研究和验证了 Cas12a 对人端粒 G4 和 TBA G4 的切割活性。我们相信这一发现将为推进 CRISPR-Cas12a 和 G4 在生物传感和生物化学中的应用开辟新途径。
