We present a case of a human cutaneous infection caused by in a 19-year-old male on adalimumab. While on a trip to Israel, the patient sustained a left knee abrasion after a fall while hiking, and subsequently went swimming in the Red Sea. The patient gradually developed a large, non-healing, erythematous, ulcerated plaque on the left knee. Initial biopsy and tissue cultures were negative for infection, but due to a high suspicion for infection, further diagnostic testing was conducted. Broad range polymerase chain reaction and next-generation sequencing was performed, and 28S rDNA sequencing was positive for Neosartorya hiratsukae, a rare infectious agent in humans. This case highlights the importance of considering uncommon sources, such as fungal etiologies, in the differential diagnosis of non-resolving lesions, particularly in immunosuppressed patients. Furthermore, this case emphasizes the significance of advanced molecular techniques in identifying uncommon pathogens.