We report here a case of allergic fungal rhinosinusitis caused by Neosartorya hiratsukae. This fungus was never previously been isolated from any case with fungal rhinosinusitis. The identification of this agent was confirmed by comparing the nucleotide sequence of the ITS region of ribosomal DNA with that in the GenBank DNA database. Identification of N. hiratsukae on the basis of colony morphology and microscopic feature may be difficult due to similarities with a few Aspergillus species. Scanning electron microscopy or DNA sequence analysis is essential for the accurate identification.