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化学发光法测定生物介质中的总氮:与参考方法的比较

[Determination of total nitrogen in biological milieux by chemoluminescence: a comparison with the reference method].

作者信息

Arock M, Desnault H, Viars P, Guillosson J J

出版信息

Ann Biol Clin (Paris). 1985;43(6):872-4.

PMID:3938626
Abstract

An assay technique for the chemiluminescent estimation of total nitrogen in biological samples have been used for two hundred urines and gastric fluids, and results compared with those given by the traditional Kjeldahl method. Chemiluminescence is accurate, reproducible, and appear to be well correlated with Kjeldahl assay technique. Without doubt, it is advantageous to the point of samples manipulation, that need no dangerous compounds, and by the way of her swiftness and her possible automation. Lastly, according with preliminary studies, this method seem as well practicable for solid samples as for liquid samples. This assay technique is therefore usefull in current practice to investigate nitrogen balance in every patient.

摘要

一种用于化学发光法测定生物样品中总氮的分析技术已应用于200份尿液和胃液样本,并将结果与传统凯氏定氮法的结果进行了比较。化学发光法准确、可重复,且似乎与凯氏定氮分析技术有良好的相关性。毫无疑问,就样品处理而言,它具有优势,不需要危险化合物,而且操作迅速,有可能实现自动化。最后,根据初步研究,这种方法对于固体样品和液体样品似乎同样适用。因此,这种分析技术在当前实践中对于研究每位患者的氮平衡很有用。

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