School of Chemistry and Chemical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
School of Chemistry and Chemical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 5;326:125256. doi: 10.1016/j.saa.2024.125256. Epub 2024 Oct 5.
In this work, a fluorescence chemical sensor continuous detection Al and phosphates by a Salamo-Salen-Salamo type compound (SL) was employed. The sensor continuously recognized Al and phosphates with good selectivity and fast response time, and a low limit of detection of 0.25 μΜ and 0.96 μM, at the same time accompanied by a naked-eye identification specificity. The detection mechanism of SL towards Al is due to the chelating fluorescence enhancement effect and ICT effect, and continuously towards phosphates is due to the collapse of the SL-Al and coordination interaction between Al and phosphates, by Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, other spectral characterization and DFT calculation as evidence. In addition, the sensor had good recyclability and reusability. The distribution of Al and phosphates in zebrafish cells was effectively monitored by confocal microscopy based on the good biocompatibility and tissue permeability of SL. Furthermore, the feasibility of using sensor SL to detect the content of Al and phosphate ions in certain drugs was quantitatively analyzed through experiments. It was found SL had a good result in practical application.
在这项工作中，采用了一种 Salamo-Salen-Salamo 型化合物 (SL) 作为荧光化学传感器，连续检测 Al 和磷酸盐。该传感器对 Al 和磷酸盐具有良好的选择性和快速的响应时间，检测限低至 0.25μΜ 和 0.96μM，同时具有肉眼识别的特异性。SL 对 Al 的检测机制归因于螯合荧光增强效应和 ICT 效应，而对磷酸盐的连续检测则归因于 SL-Al 的塌陷和 Al 与磷酸盐之间的配位相互作用，傅里叶变换红外光谱、X 射线光电子能谱等光谱表征和 DFT 计算为证。此外，该传感器具有良好的可回收性和可重复使用性。基于 SL 的良好生物相容性和组织通透性，通过共聚焦显微镜有效地监测了 Al 和磷酸盐在斑马鱼细胞中的分布。此外，通过实验定量分析了传感器 SL 检测某些药物中 Al 和磷酸盐离子含量的可行性。结果表明，SL 在实际应用中具有良好的效果。
