Department of Prenatal Diagnosis, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Guangxi Key Laboratory of Thalassemia Research, Nanning, Guangxi, China.
Sci Rep. 2024 Oct 10;14(1):23773. doi: 10.1038/s41598-024-74242-6.
Uterine corpus endometrial carcinoma (UCEC) is one of the most common tumours of the female reproductive system. CCAAT enhancer-binding protein alpha (CEBPA) is a member of the transcription factor family involved in regulating processes such as cell proliferation, differentiation, metabolism, and the immune response. However, the role of CEBPA in UCEC has not been clarified. Here, we performed a comprehensive analysis to explore the expression level, prognostic value, immune infiltration and biological function of CEBPA in UCEC. In this study, we found that CEBPA expression was upregulated and associated with poor prognosis in UCEC patients. KEGG and GO analyses revealed that the genes positively correlated with CEBPA were enriched primarily in immune regulation and oxidative phosphorylation. Immune infiltration analysis revealed that CEBPA is strongly correlated with immune cell infiltration in UCEC. RT-qPCR indicated that CEBPA may regulate the OXPHOS level in Ishikawa cells. CCK-8, cell cycle, Transwell and scratch wound healing assays revealed that CEBPA promoted Ishikawa cell proliferation, invasion and migration. In addition, PPI and survival analyses suggested that CEBPG may be a potential target of CEBPA in UCEC. These results demonstrated that CEBPA may be a potential therapeutic target in UCEC.
子宫体子宫内膜癌（UCEC）是女性生殖系统最常见的肿瘤之一。CCAAT 增强子结合蛋白α（CEBPA）是转录因子家族的一员，参与调节细胞增殖、分化、代谢和免疫反应等过程。然而，CEBPA 在 UCEC 中的作用尚未阐明。在这里，我们进行了全面的分析，以探讨 CEBPA 在 UCEC 中的表达水平、预后价值、免疫浸润和生物学功能。在这项研究中，我们发现 CEBPA 的表达上调与 UCEC 患者的不良预后相关。KEGG 和 GO 分析显示，与 CEBPA 呈正相关的基因主要富集在免疫调节和氧化磷酸化中。免疫浸润分析显示，CEBPA 与 UCEC 中免疫细胞浸润呈强相关。RT-qPCR 表明 CEBPA 可能调节 Ishikawa 细胞中的 OXPHOS 水平。CCK-8、细胞周期、Transwell 和划痕愈合实验表明，CEBPA 促进了 Ishikawa 细胞的增殖、侵袭和迁移。此外，PPI 和生存分析表明，CEBPG 可能是 UCEC 中 CEBPA 的潜在靶点。这些结果表明，CEBPA 可能是 UCEC 中的一个潜在治疗靶点。
