Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, The Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, Changsha Medical University, Changsha, People's Republic of China.
Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, The Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, Changsha Medical University, 1501 Leifeng Road, 410219, Changsha, People's Republic of China.
Sci Rep. 2024 Oct 10;14(1):23742. doi: 10.1038/s41598-024-75636-2.
B cells play a key role in humoral immune responses by producing antibodies. Although there are numerous research on memory B cells definition markers and cytokines on B cell development, different studies have yielded contradictory conclusions due to species studied, the different cells and stimulating agents used. In the current study, we conducted a detailed characterization of B cells in human CBMCs, PBMCs and tonsil, including expression of Igs, activation and memory markers. Furthermore, we found that considerable amounts of IgA and IgG were expressed by CD27 B cells. These "Atypical" memory B cells corresponded to approximately 50% of IgG and IgAB cells in blood, this proportion even reached 90% in tonsil. In addition, we investigated the effect of IL-21 and TGF-β1 on the membrane-bound form and secreted form of Igs using PBMCs and purified blood B cells. There were actual differences between the effect of cytokines on Igs secretion and surface expression. Our study will be helpful to advance the knowledge and understanding of humoral memory.
B 细胞通过产生抗体在体液免疫反应中发挥关键作用。尽管有许多关于记忆 B 细胞定义标记物和细胞因子在 B 细胞发育中的作用的研究，但由于研究的物种、使用的不同细胞和刺激剂，不同的研究得出了相互矛盾的结论。在本研究中，我们对人 CBMC、PBMC 和扁桃体中的 B 细胞进行了详细的特征描述，包括 Ig 的表达、激活和记忆标记物。此外，我们发现 CD27 B 细胞表达相当数量的 IgA 和 IgG。这些“非典型”记忆 B 细胞约占血液中 IgG 和 IgA+B 细胞的 50%，在扁桃体中甚至达到 90%。此外，我们研究了 IL-21 和 TGF-β1 对 PBMC 和纯化的血液 B 细胞中 Ig 膜结合形式和分泌形式的影响。细胞因子对 Ig 分泌和表面表达的影响存在实际差异。我们的研究将有助于提高对体液记忆的认识和理解。
