Müller H E, Aleksic S, Elsarnagawy D, Al-Mashhadi M A, Bockemühl J
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1985 Dec;182(1):58-63.
23 samples of effluent of the wastewater treatment plant of Jeddah/Saudi Arabia were investigated between September 1983 and November 1984. A total of 395 strains of gram-negative fermentative bacteria were isolated and differentiated at species level. Neither vibrios, salmonellae, or shigellae were detected notwithstanding of enrichment procedures used. Furthermore, the occurrence of E. coli producing heat labile enterotoxin (LT) was studied using both Biken test and GM1-ELISA. The Biken test produced many false positive results which could not been confirmed by the more specific GM1-ELISA. All strains of E. coli investigated were negative. Therefore, the Biken test seems not to be a recommended method for detection of E. coli LT.
1983年9月至1984年11月期间，对沙特阿拉伯吉达污水处理厂的23份废水样本进行了调查。共分离出395株革兰氏阴性发酵菌，并在种水平上进行了鉴别。尽管采用了富集程序，但未检测到弧菌、沙门氏菌或志贺氏菌。此外，使用比肯试验和GM1-ELISA研究了产热不稳定肠毒素（LT）的大肠杆菌的发生情况。比肯试验产生了许多假阳性结果，而更具特异性的GM1-ELISA无法证实这些结果。所有调查的大肠杆菌菌株均为阴性。因此，比肯试验似乎不是检测大肠杆菌LT的推荐方法。
