Hirata J, Kaneko S, Nishimura J, Motomura S, Ibayashi H
Acta Haematol. 1985;74(4):189-94. doi: 10.1159/000206216.
The effects of various human sera, platelet lysates and platelet-derived growth factor (PDGF) on the proliferation of human bone marrow-derived fibroblastoid colony-forming cells (CFU-F) were examined. We obtained nearly identical growth curves of fibroblastoid colonies with sera, platelet lysates and PDGF as stimulants and concluded that PDGF was a main growth factor for CFU-F in human serum. In contrast to colony size, CFU-F number was irrelevant to the concentration of PDGF. Removal of culture medium containing hemopoietic cells after short-term incubation of bone marrow cells reduced both colony number and size in CFU-F cultures. When each of bone marrow-conditioned medium (BMCM), peripheral blood mononuclear cells (MNC) and phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM) was added to the cultures, CFU-F number and colony size recovered. The role of PDGF and the factors present in BMCM, MNC and PHA-LCM in the growth of CFU-F and their precursor cells were discussed.
研究了各种人血清、血小板裂解物和血小板衍生生长因子（PDGF）对人骨髓来源的成纤维细胞样集落形成细胞（CFU-F）增殖的影响。我们以血清、血小板裂解物和PDGF作为刺激物，获得了几乎相同的成纤维细胞样集落生长曲线，并得出结论，PDGF是人类血清中CFU-F的主要生长因子。与集落大小不同，CFU-F的数量与PDGF的浓度无关。骨髓细胞短期培养后去除含有造血细胞的培养基，会减少CFU-F培养物中的集落数量和大小。当将骨髓条件培养基（BMCM）、外周血单个核细胞（MNC）和植物血凝素刺激的白细胞条件培养基（PHA-LCM）分别添加到培养物中时，CFU-F的数量和集落大小得以恢复。讨论了PDGF以及BMCM、MNC和PHA-LCM中存在的因子在CFU-F及其前体细胞生长中的作用。
