Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China.
Institute of Clinical Pharmacology, Central South University; Hunan Key Laboratory of Pharmacogenetics, Changsha, 410078, China.
Pharmacogenomics. 2024;25(10-11):461-468. doi: 10.1080/14622416.2024.2404819. Epub 2024 Oct 11.
To investigate the associations between genetic polymorphisms in immunorelated genes and PBMC-induced cytotoxicity to breast cancer cell with the treatment of trastuzumab . Trastuzumab-mediated cytotoxicity of peripheral blood mononuclear cells (PBMC) from 148 healthy donors and 13 BC patients was analyzed by flow cytometry. 16 SNPs in 7 immunorelated genes were genotyped by Sequenom Mass Array Genotype Platform. Cytotoxicity in the trastuzumab treated PBMCs were significantly higher than those of the basal group. A wide variability in trastuzumab-mediated cytotoxicity was observed, and PBMC from individuals with the rs16859030 T genotype generated increased cytotoxicity than those with the CC genotype. The rs16859030 polymorphism affects trastuzumab-mediated cytotoxicity .
为了研究免疫相关基因的遗传多态性与曲妥珠单抗治疗乳腺癌细胞的外周血单个核细胞（PBMC）诱导细胞毒性之间的关系。通过流式细胞术分析了 148 名健康供体和 13 名 BC 患者的 PBMC 对曲妥珠单抗的细胞毒性。通过 Sequenom MassArray Genotype 平台对 7 个免疫相关基因中的 16 个 SNP 进行了基因分型。与基础组相比，曲妥珠单抗处理的 PBMC 的细胞毒性明显更高。曲妥珠单抗介导的细胞毒性存在广泛的可变性，并且具有 rs16859030T 基因型的个体的 PBMC 产生的细胞毒性比具有 CC 基因型的个体更高。rs16859030 多态性影响曲妥珠单抗介导的细胞毒性。
