Cao Minghui, Cui Xuejiao, Chen Yadong, Yan Wenwen, Zeng Weimin, Zhang Yanlong, Jia Xiangqian
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Int J Biol Macromol. 2024 Nov;281(Pt 1):136409. doi: 10.1016/j.ijbiomac.2024.136409. Epub 2024 Oct 9.
In our research, a novel polysaccharide (named SSP-3a) with uniform molecular weight was extracted from Scutellaria baicalensis stem-leaf. The structural analysis revealed that SSP-3a was an acidic polysaccharide with a heavy average molecular weight of 1.83 × 10 Da. By HPLC, the primary constituents of SSP-3a were mannose (11.60 %), glucuronic acid (42.99 %), glucose (23.43 %), and xylose (22.04 %). According to FT-IR and H NMR analysis, it was confirmed to be a β-configuration pyranose with a CO stretching vibrational peak. The immunomodulation results also showed that SSP-3a not only significantly promoted RAW264.7 cell proliferation and phagocytosis, but also stimulated the release of NO and cytokines. Furthermore, mechanistic studies suggested that SSP-3a had the ability to trigger MAPKs and NF-κB immunological signaling pathways via TLR4 receptors. The findings suggested that SSP-3a might be a beneficial active component for the food and pharmaceutical industries.
在我们的研究中，从黄芩茎叶中提取出了一种分子量均一的新型多糖（命名为SSP-3a）。结构分析表明，SSP-3a是一种酸性多糖，重均分子量为1.83×10 Da。通过高效液相色谱法（HPLC）分析，SSP-3a的主要成分是甘露糖（11.60%）、葡萄糖醛酸（42.99%）、葡萄糖（23.43%）和木糖（22.04%）。根据傅里叶变换红外光谱（FT-IR）和核磁共振氢谱（H NMR）分析，证实其为具有CO伸缩振动峰的β构型吡喃糖。免疫调节结果还表明，SSP-3a不仅能显著促进RAW264.7细胞增殖和吞噬作用，还能刺激一氧化氮（NO）和细胞因子的释放。此外，机制研究表明，SSP-3a能够通过Toll样受体4（TLR4）触发丝裂原活化蛋白激酶（MAPKs）和核因子κB（NF-κB）免疫信号通路。这些研究结果表明，SSP-3a可能是食品和制药行业中一种有益的活性成分。
