Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
BMC Biotechnol. 2024 Oct 11;24(1):79. doi: 10.1186/s12896-024-00901-1.
Sepsis is an inevitable stage of bacterial invasion characterized by an uncontrolled inflammatory response resulting in a syndrome of multiorgan dysfunction. Most conventional antibiotics used to treat sepsis are efficacious, but they have undesirable side effects. The green synthesised Ag NPs were synthesized by 5 g of the earthworm extract dissolved in a volume of 500mL of distilled water and then added to 2,500 mL aqueous solution of 1mM silver nitrate at 40 °C. After 4 h, the mixture was then allowed to dry overnight at 60 °C. Later, Ag NPs were washed and collected. They were characterized by X-ray diffraction, ultraviolet-visible spectroscopy, and transmission electron microscopy. Sepsis model as induced by feces-intraperitoneal injection method. Eighteen male mice were assigned into three main groups: the control group, the sepsis-model group, and the Ag NPs-treated group. The control group received a single oral dose of distilled water and, after two days, intraperitoneally injected with 30% glycerol in phosphate buffer saline. The Sepsis-model group received a single oral dose of distilled water. Ag NPs - The treated group received a single oral dose of 5.5 mg/kg of Ag NPs. After two days, the sepsis-model group and Ag NPs-treated group were intraperitoneally injected with 200 µL of faecal slurry. Ag NPs treatment in septic mice significantly decreased liver enzyme activities, total protein, and serum albumin. Moreover, Ag NPs significantly enhanced kidney function, as indicated by a significant decrease in the levels of creatinine, urea, and uric acid. In addition, Ag NPs showed a powerful antioxidant effect via the considerable reduction of malondialdehyde and nitric oxide levels and the increase in antioxidant content. The histopathological investigation showed clear improvement in hepatic and kidney architecture. Our findings demonstrate the protective efficacy of biogenic Ag NPs against sepsis-induced liver and kidney damage.
脓毒症是细菌入侵的必然阶段，其特征是失控的炎症反应导致多器官功能障碍综合征。大多数用于治疗脓毒症的传统抗生素都有效，但它们有不良的副作用。绿色合成的 Ag NPs 是通过将 5 g 的蚯蚓提取物溶解在 500 mL 的蒸馏水中，然后在 40°C 下加入 2500 mL 1mM 硝酸银的水溶液来合成的。4 小时后，混合物在 60°C 下干燥过夜。然后，Ag NPs 被洗涤和收集。它们的特征是通过 X 射线衍射、紫外可见光谱和透射电子显微镜。通过粪便腹腔注射法诱导脓毒症模型。将 18 只雄性小鼠分为三组：对照组、脓毒症模型组和 Ag NPs 治疗组。对照组给予单次口服蒸馏水，两天后腹腔内注射磷酸盐缓冲生理盐水 30%甘油。脓毒症模型组给予单次口服蒸馏水。Ag NPs 治疗组给予单次口服 5.5mg/kg 的 Ag NPs。两天后，脓毒症模型组和 Ag NPs 治疗组腹腔内注射 200 μL 粪便浆。Ag NPs 治疗脓毒症小鼠显著降低了肝酶活性、总蛋白和血清白蛋白。此外，Ag NPs 显著增强了肾功能，表现为肌酐、尿素和尿酸水平显著降低。此外，Ag NPs 通过显著降低丙二醛和一氧化氮水平和增加抗氧化剂含量表现出强大的抗氧化作用。组织病理学研究表明，肝和肾结构有明显改善。我们的研究结果表明，生物合成的 Ag NPs 对脓毒症引起的肝和肾损伤具有保护作用。
