菜粉蝶（鳞翅目：粉蝶科）染色体水平基因组组装。

A chromosomal-level genome assembly of Corcyra cephalonica Stainton (Lepidoptera: Pyralidae).

机构信息

Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang, Jurong, 212400, China.

College of Life Sciences, Xinyang Normal University, Xinyang, 464000, China.

出版信息

Sci Data. 2024 Oct 11;11(1):1118. doi: 10.1038/s41597-024-03967-w.

DOI:10.1038/s41597-024-03967-w

PMID:39394231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11470032/

Abstract

The family Pyralidae is a large and important group within the superfamily Pyraloidea, consisting of more than 5,900 described species. The rice moth Corcyra cephalonica is a noteworthy pest affecting stored grains and products. However, the absence of high-quality genomes hinders our comprehension of the ecology of C. cephalonica. Herein, we propose a chromosome-level genome of C. cephalonica achieved through integrating Nanopore, Illumina, and Hi-C reads. The genome of 453.37 Mb in size consists of 98 scaffolds and 154 contigs, with a scaffold N50 size of 16.67 Mb. The majority of contigs (99.36%, 450.45 Mb) were effectively anchored to 29 chromosomes. The BUSCO analysis of the assembly revealed a completeness of 99.0% (n = 1,367), identifying 98.6% single-copy BUSCO genes and 0.4% duplicated BUSCO genes. Furthermore, the genome contains 48.74% (220.96 Mb) repeat elements and encompasses 14,135 predicted protein-coding genes. This study offers a valuable genomic foundation for advancing our comprehension of the biology of C. cephalonica.

摘要

麦蛾科是鳞翅目麦蛾总科中一个庞大而重要的科，包含超过 5900 个已描述的物种。玉米象 Corcyra cephalonica 是一种影响储粮和产品的重要害虫。然而，由于缺乏高质量的基因组，我们对 C. cephalonica 的生态学了解有限。在此，我们通过整合纳米孔、Illumina 和 Hi-C 读取提出了 C. cephalonica 的染色体水平基因组。该基因组大小为 453.37Mb，由 98 个支架和 154 个 contigs 组成，支架 N50 大小为 16.67Mb。大多数 contigs（99.36%，450.45Mb）有效地锚定在 29 条染色体上。组装的 BUSCO 分析显示完整性为 99.0%（n=1,367），鉴定出 98.6%的单拷贝 BUSCO 基因和 0.4%的重复 BUSCO 基因。此外，基因组包含 48.74%（220.96Mb）的重复元件，包含 14,135 个预测的蛋白质编码基因。这项研究为深入了解 C. cephalonica 的生物学提供了有价值的基因组基础。