Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Frederiksberg C, Denmark.
Laboratory of Veterinary Pathology and Nanopathology, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, 100193 Beijing, P. R. China.
J Appl Microbiol. 2024 Nov 4;135(11). doi: 10.1093/jambio/lxae259.
To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains.
The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres.
This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.
开发并评估载有乳链菌肽的壳聚糖/海藻酸钠（CS/SA）微球，作为一种针对金黄色葡萄球菌菌株的改良抗菌药物输送系统。
采用改良的水包油乳液交联法制备微球，得到粒径为 1-8µm 的球形颗粒，表面电荷为-7.92±5.09mV，通过扫描电子显微镜（SEM）和 Zetasizer 分析得到证实。乳链菌肽的包封效率（EE）和载药量（LC）分别为 87.60%±0.43%和 1.99%±0.01%。48 小时的体外释放研究表明，乳链菌肽呈现出一种控制释放模式，符合 Korsmeyer-Peppas 模型，在 37°C 和碱性 pH 值下释放速率更高。抗菌试验表明，载有乳链菌肽的 CS/SA 微球比游离乳链菌肽具有更高的疗效，最小抑菌浓度值降低了 50%。共聚焦激光扫描显微镜（CLSM）、SEM 和透射电子显微镜显示，微球对细菌膜造成了显著的损伤和细胞破坏。
本研究强调了载有乳链菌肽的 CS/SA 微球作为一种创新的抗菌药物输送系统的潜力，该系统具有改善的稳定性和针对金黄色葡萄球菌的抗菌功效，解决了单独应用乳链菌肽所存在的局限性。
