Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
Arch Microbiol. 2024 Oct 12;206(11):431. doi: 10.1007/s00203-024-04156-4.
Staphylococcus aureus-induced mastitis is a serious disease in dairy bovine, with no currently effective treatment. Antibiotics demonstrate certain therapeutic potency in dairy husbandry; they generate drug-resistant bacteria, thereby harming public health. LncRNAs and mA have been verified as potential targets in infectious diseases and have powerful regulatory capabilities. However, the biological regulation of lncRNAs with mA modification in mastitis needs further investigation. This study aims to determine the mA-modified lncRNAs in bovine mammary epithelial cells and their diversity during S. aureus induction. Heat-inactivated S. aureus was used to develop the cell injury model, and we subsequently found low cell viability and different mA modification levels. Our analysis of mA-modified lncRNA profiles through MeRIP-seq revealed significant differences in 140 peaks within 130 lncRNAs when cells were injured by S. aureus. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these differential mA-modified lncRNAs were mainly enriched in the WNT pathway, and their functions were associated with amino acid metabolism, lipid translocation, and metalloproteinase activity. Here, we report for the first time lncRNAs with mA modification in regulating S. aureus infection, revealing potential mechanisms and targets of infectious diseases, such as mastitis, from an epigenetics perspective.
金黄色葡萄球菌诱导的乳腺炎是奶牛的一种严重疾病，目前尚无有效的治疗方法。抗生素在奶牛养殖中具有一定的治疗效力；但它们会产生耐药菌，从而危害公共健康。lncRNAs 和 mA 已被证实为传染病的潜在靶点，具有强大的调节能力。然而，乳腺炎中 lncRNA 的 mA 修饰的生物学调节作用仍需进一步研究。本研究旨在确定金黄色葡萄球菌诱导牛乳腺上皮细胞中的 mA 修饰 lncRNA 及其多样性。我们使用热灭活的金黄色葡萄球菌来建立细胞损伤模型，随后发现细胞活力降低和 mA 修饰水平不同。通过 MeRIP-seq 分析 mA 修饰的 lncRNA 图谱，我们发现金黄色葡萄球菌损伤细胞时，在 130 个 lncRNA 中有 140 个峰的 mA 修饰存在显著差异。此外，GO 和 Kyoto Encyclopedia of Genes and Genomes 富集分析表明，这些差异的 mA 修饰 lncRNA 主要富集在 WNT 通路中，其功能与氨基酸代谢、脂质转运和金属蛋白酶活性有关。本研究首次报道了 mA 修饰在调节金黄色葡萄球菌感染中的 lncRNA，从表观遗传学角度揭示了乳腺炎等传染病的潜在机制和靶点。
