Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Antiviral Res. 2022 Sep;205:105382. doi: 10.1016/j.antiviral.2022.105382. Epub 2022 Jul 12.
Japanese encephalitis (JE) is a mosquito-borne flavivirus infection named Japanese Encephalitis Virus (JEV), prevalent in Asia-pacific countries, requires an accurate and rapid diagnosis to contain the outbreak of the disease. In cases of low viral load in early-stage infections, this task becomes difficult. Therefore, we have developed a surface-enhanced Raman spectroscopy (SERS) based biosensor for rapid, sensitive, and early-stage detection of JE antigen. In this work, silver nanoparticles were deposited over a glass coverslip and used as a substrate for designing the sensing platform. Silver Nanoparticles have good metallic properties and plasmon activity. Therefore, it amplifies the Raman signals and provides a suitable surface for the SERS substrate. The developed platform has been used for the detection of the Japanese encephalitis virus (JEV). The fabricated sensor shows a linear response from 5 ng/mL to 80 ng/mL with a limit of detection (LoD) of ∼7.6 ng/mL. Therefore, this method could be a significant addition to the diagnostic modalities for early, sensitive, and specific diagnoses of JE antigen even at the nanogram level.
日本脑炎 (JE) 是一种由蚊子传播的黄病毒感染，被命名为日本脑炎病毒 (JEV)，在亚太国家流行，需要准确和快速的诊断来控制疾病的爆发。在感染早期病毒载量较低的情况下，这项任务变得很困难。因此，我们开发了一种基于表面增强拉曼光谱 (SERS) 的生物传感器，用于快速、灵敏和早期检测 JE 抗原。在这项工作中，银纳米粒子沉积在玻璃盖玻片上，用作设计传感平台的基底。银纳米粒子具有良好的金属性质和等离子体活性。因此，它可以放大拉曼信号，并为 SERS 基底提供合适的表面。所开发的平台已用于检测日本脑炎病毒 (JEV)。所制备的传感器在 5ng/mL 至 80ng/mL 的范围内表现出线性响应，检测限 (LoD) 约为 7.6ng/mL。因此，即使在纳克级水平，这种方法也可以为 JE 抗原的早期、灵敏和特异性诊断提供重要的诊断方式。
