Tianjian Laboratory of Advanced Biomedical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China; Henan Medical School, Zhengzhou University, Zhengzhou 450000, Henan, China.
Tianjian Laboratory of Advanced Biomedical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Oct 15;1247:124335. doi: 10.1016/j.jchromb.2024.124335. Epub 2024 Oct 9.
As a medicinal plant, Cirsium setosum has excellent procoagulant effects and has long been used as a cure for hemoptysis, epistaxis, uremia and metrorrhagia caused by blood heat. However, the key medicinal part of C. setosum, as well as the biologically active substances that play a major role, are not known. In this study, the aboveground, underground and whole grass portions of C. setosum were subjected to a coagulation comparison experiment to determine the primary active procoagulant compounds. The main active procoagulant compounds of C. setosum were then screened using a comparative metabolomics analysis between aboveground and underground. Network pharmacology analysis was used to construct the "active ingredient-disease target-pathway" network. Finally, molecular docking was used to verify the binding ability and affinity between the key active ingredients obtained from the screening and the targets. The results indicated that the aboveground part of C. setosum could significantly shorten activated partial thromboplastin time (APTT) and that this part exerts substantial procoagulant effects. The total phenol, total flavonoid and total alkaloid content of the aboveground part was measured to be higher than those of the underground part and whole grass. Furthermore, comparative metabolomics analysis as well as the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database and literature search screening yielded 10 active substances, including naringenin, guanine, 2,4-di-tert-butylphenol, calycosin-7-O-beta-D-glucoside, flavone, vitexin, and tiliroside, which may be related to the coagulation-promoting properties of C. setosum and its therapeutic effects on coagulation-related disorders. Network pharmacological analysis revealed that C. setosum may exert procoagulant effects mainly through tiliroside, calycosin-7-O-beta-D-glucoside, and flavone, which act on key target proteins, such as SRC, PRKACA, EGFR, AKT1, MAPK3, and GSK3B. In summary, C. setosum exerts its procoagulant and therapeutic effects on coagulation-related diseases through multiple compounds, targets, and pathways.
作为一种药用植物，蓟具有极好的促凝血作用，长期以来一直被用作治疗血热引起的咯血、鼻出血、尿毒症和月经过多。然而，蓟的关键药用部位以及起主要作用的生物活性物质尚不清楚。在这项研究中，对蓟的地上部分、地下部分和全草部分进行了凝血比较实验，以确定主要的活性促凝血化合物。然后，使用地上部分和地下部分之间的比较代谢组学分析来筛选蓟的主要活性促凝血化合物。网络药理学分析用于构建“活性成分-疾病靶标-途径”网络。最后，分子对接用于验证从筛选中获得的关键活性成分与靶标之间的结合能力和亲和力。结果表明，蓟的地上部分可以显著缩短活化部分凝血活酶时间（APTT），并且具有明显的促凝血作用。地上部分的总酚、总黄酮和总生物碱含量均高于地下部分和全草。此外，比较代谢组学分析以及中药系统药理学数据库和分析平台（TCMSP）数据库和文献搜索筛选出 10 种活性物质，包括柚皮素、鸟嘌呤、2,4-二叔丁基苯酚、毛蕊异黄酮-7-O-β-D-葡萄糖苷、黄酮、牡荆素、和桃叶珊瑚苷，它们可能与蓟的促凝血特性及其对凝血相关疾病的治疗作用有关。网络药理学分析表明，蓟可能主要通过桃叶珊瑚苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷和黄酮发挥促凝血作用，作用于关键靶蛋白，如 SRC、PRKACA、EGFR、AKT1、MAPK3 和 GSK3B。总之，蓟通过多种化合物、靶标和途径发挥其促凝血和治疗凝血相关疾病的作用。
