Jiang Xiaojing, Zhu Shaoxuan, Song Jinying, Li Xingwei, Li Chengbo, Hou Guige, Gao Zhongfei
Key Laboratory of Medical Antibacterial Materials of Shandong Province, School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
Key Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Science, Shandong Agricultural University, Tai'an 271018, China.
Pharmaceutics. 2025 May 17;17(5):660. doi: 10.3390/pharmaceutics17050660.
: (commonly known as thistle) is a traditional Chinese medicinal plant with significant therapeutic potential, exhibiting hemostatic, antioxidant, and wound-healing properties. Electrospinning offers a versatile platform for fabricating nanoscale scaffolds with tunable functionality, making them ideal for drug delivery and tissue engineering. : In this study, a bioactive extract from thistle was obtained and incorporated into a thermosensitive triblock copolymer (PNNS) and polycaprolactone (PCL) to develop a multifunctional nanofibrous scaffold for enhanced wound healing. The prepared nanofibers were thoroughly characterized using Fourier-transform infrared spectroscopy (FTIR), contact angle measurements, thermogravimetric analysis (TGA), and tensile fracture testing to assess their physicochemical properties. : Notably, the inclusion of PNNS imparted temperature-responsive behavior to the scaffold, enabling controlled deformation in response to thermal stimuli-a feature that may facilitate wound contraction and improve scar remodeling. Specifically, the scaffold demonstrated rapid shrinkage at a physiological temperature (38 °C) within minutes while maintaining structural integrity at ambient conditions (20 °C). In vitro studies confirmed the thistle extract's potent antioxidant activity, while in vivo experiments revealed their effective hemostatic performance in a liver bleeding model when delivered via the composite nanofibers. Thistle extract and skin temperature-responsive contraction reduced the inflammatory outbreak at the wound site and promoted collagen deposition, resulting in an ideal wound-healing rate of above 95% within 14 days. : The integrated strategy that combines mechanical signals, natural extracts, and electrospinning nanotechnology offers a feasible design approach and significant technological advantages with enhanced therapeutic efficacy.
蓟（俗称刺儿菜）是一种具有显著治疗潜力的传统中药材，具有止血、抗氧化和伤口愈合特性。静电纺丝为制造具有可调功能的纳米级支架提供了一个多功能平台，使其成为药物递送和组织工程的理想选择。在本研究中，从蓟中获得了一种生物活性提取物，并将其掺入热敏性三嵌段共聚物（PNNS）和聚己内酯（PCL）中，以开发一种用于增强伤口愈合的多功能纳米纤维支架。使用傅里叶变换红外光谱（FTIR）、接触角测量、热重分析（TGA）和拉伸断裂测试对制备的纳米纤维进行了全面表征，以评估其物理化学性质。值得注意的是，PNNS的加入赋予了支架温度响应行为，使其能够响应热刺激而发生可控变形——这一特性可能有助于伤口收缩并改善瘢痕重塑。具体而言，该支架在生理温度（38°C）下几分钟内就表现出快速收缩，同时在环境条件（20°C）下保持结构完整性。体外研究证实了蓟提取物具有强大的抗氧化活性，而体内实验表明，当通过复合纳米纤维递送时，它们在肝出血模型中具有有效的止血性能。蓟提取物和皮肤温度响应性收缩减少了伤口部位的炎症爆发，并促进了胶原蛋白沉积，在14天内实现了95%以上的理想伤口愈合率。将机械信号、天然提取物和静电纺丝纳米技术相结合的综合策略提供了一种可行的设计方法，并具有显著的技术优势，可提高治疗效果。
