Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Department of Livestock and Poultry Products Testing, Beijing General Station of Animal Husbandry, Beijing, 100107, China.
Cryobiology. 2024 Dec;117:104979. doi: 10.1016/j.cryobiol.2024.104979. Epub 2024 Oct 23.
Cryopreservation causes higher reactive oxygen species (ROS) concentrations, leading to oxidative stress and lipid peroxidation damaging sperm, and using antioxidants can improve semen quality after freeze-thaw. Natural astaxanthin (ASTA) can be inserted into cell membranes and its antioxidant properties are stronger than other antioxidants. We aimed to investigate the effects of ASTA supplementation in the Beltsville Poultry Semen Extender (BPSE) on post-thaw rooster semen quality and to explore the potential mechanism of rooster semen quality change. The qualifying semen ejaculates collected from 30 adult male Jinghong No. 1 laying hen breeder roosters (65 wk old) were pooled, divided into four aliquots, and diluted with BPSE having different levels of ASTA (0, 0.5, 1, or 2 μg/mL). Treated semen was cryopreserved and kept in liquid nitrogen. The entire experiment was replicated three times independently. Sperm viability, motility, curvilinear velocity, amplitude of lateral head displacement, straightness, plasma membrane integrity, and acrosome integrity were observed to be highest (P < 0.05) with 1 μg/mL ASTA at freeze-thawing. Higher (P < 0.05) antioxidant enzyme (CAT-like, SOD) activities and free radical (·OH, O2) scavenging ability, less ROS and malondialdehyde (MDA) concentrations were recorded with the addition of appropriate concentrations of ASTA compared to control. In addition, the levels of mitochondrial membrane potential (MMP), adenosine triphosphate (ATP), and lactate dehydrogenase (LDH) in the 1 μg/mL ASTA group improved compared to the control group, and decreased the amount of AIF protein level but increased the Bcl-2 protein level (P < 0:05). Collectively, these results demonstrate that adding ASTA in the BPSE promoted rooster freeze-thaw sperm quality, which may be related to reducing ROS levels, protecting the antioxidant defense system, preventing lipid peroxidation, improving mitochondrial structural and functional integrity, and inhibiting sperm apoptosis.
冷冻保存会导致活性氧（ROS）浓度升高，从而导致氧化应激和脂质过氧化损伤精子，而使用抗氧化剂可以改善冷冻-解冻后的精液质量。天然虾青素（ASTA）可以插入细胞膜，其抗氧化性能强于其他抗氧化剂。我们旨在研究 AST 在贝尔茨维尔家禽精液稀释液（BPSE）中对冷冻解冻后公鸡精液质量的影响，并探讨公鸡精液质量变化的潜在机制。从 30 只成年雄性景红 1 号蛋鸡种公鸡（65 周龄）收集符合条件的精液射精液，混合后分为四等份，分别用含有不同浓度 AST（0、0.5、1 或 2μg/ml）的 BPSE 稀释。处理后的精液进行冷冻保存并保存在液氮中。整个实验独立重复三次。结果表明，在冷冻-解冻时，1μg/ml AST 的精子存活率、活力、曲线速度、头部侧向位移幅度、直线度、质膜完整性和顶体完整性最高（P<0.05）。与对照组相比，添加适当浓度的 AST 可提高抗氧化酶（CAT 样、SOD）活性和自由基（·OH、O2）清除能力，降低 ROS 和丙二醛（MDA）浓度（P<0.05）。此外，与对照组相比，1μg/ml AST 组的线粒体膜电位（MMP）、三磷酸腺苷（ATP）和乳酸脱氢酶（LDH）水平有所提高，AIF 蛋白水平降低，Bcl-2 蛋白水平升高（P<0.05）。综上所述，这些结果表明，在 BPSE 中添加 AST 可提高公鸡冷冻-解冻精液质量，这可能与降低 ROS 水平、保护抗氧化防御系统、防止脂质过氧化、改善线粒体结构和功能完整性以及抑制精子凋亡有关。
