School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, 453003, PR China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, PR China.
School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, 453003, PR China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, PR China.
Gene. 2025 Jan 15;933:148994. doi: 10.1016/j.gene.2024.148994. Epub 2024 Oct 11.
The green peach aphid (GPA), Myzus persicae (Sulzer), is a serious agricultural pest with a worldwide distribution and a vector of over 100 plant viruses. Various pathways, such as the mitogen-activated protein kinase (MAPK) cascades, play pivotal roles in signaling plant defense against pest attack, and circular RNAs (circRNAs) regulate the expression of mRNAs in response to pest attack. However, the mechanism underlying peach (Prunus persica) response to GPA attack remains unclear. The present study initially identified and characterized 316 circRNAs and 18 PpMAPKs from healthy and GPA-infested peach leaves by whole-transcriptome sequencing and predicted the differentially expressed circRNAs (DECs) after GPA infestation. PCR and Sanger sequencing confirmed the presence of six DECs in peach samples. Besides, RNA sequencing analysis detected 13 DECs, including 5 upregulated and 8 downregulated ones, in peach in response to the GPA attack. Gene ontology (GO) enrichment analysis indicated that specific DECs play crucial roles in the MAPK signaling pathway, and qRT-PCR revealed that GPA infestation altered the expression patterns of PpMAPKs. Finally, five circRNAs, three microRNA (miRNAs), and two MAPK target genes were identified to interact as a network and perform critical roles in modulating the MAPK pathway in the peach during GPA infestation.
绿盲蝽（GPA），桃蚜（Myzus persicae），是一种分布广泛的严重农业害虫，也是 100 多种植物病毒的载体。各种途径，如丝裂原活化蛋白激酶（MAPK）级联反应，在植物防御害虫攻击的信号转导中起着关键作用，而环状 RNA（circRNA）则调节 mRNA 的表达以响应害虫的攻击。然而，桃（Prunus persica）对 GPA 攻击的反应机制尚不清楚。本研究通过全转录组测序首次鉴定和表征了健康和 GPA 感染桃叶中的 316 个 circRNA 和 18 个 PpMAPKs，并预测了 GPA 感染后差异表达的 circRNAs（DECs）。PCR 和 Sanger 测序证实了桃样品中六种 DECs 的存在。此外，RNA 测序分析检测到桃对 GPA 攻击的 13 个 DECs，包括 5 个上调和 8 个下调。基因本体（GO）富集分析表明，特定的 DECs 在 MAPK 信号通路中起着关键作用，qRT-PCR 显示 GPA 感染改变了 PpMAPKs 的表达模式。最后，鉴定出五个 circRNAs、三个 microRNA（miRNA）和两个 MAPK 靶基因相互作用形成网络，在 GPA 感染期间对调节桃中 MAPK 途径发挥关键作用。
