加蓬女性中高危型人乳头瘤病毒基因型和病毒载量的流行与鳞状细胞炎症相关。

Prevalence of high-risk human papillomavirus genotypes and viral load correlated with squamous cell inflammation among women in Gabon.

机构信息

National Public Health Laboratory, Libreville, Gabon.

Catholic University of Central Africa, Yaoundé, Cameroon.

出版信息

BMC Womens Health. 2024 Oct 12;24(1):561. doi: 10.1186/s12905-024-03403-6.

DOI:10.1186/s12905-024-03403-6

PMID:39396007

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11475162/

Abstract

BACKGROUND

High-risk genotypes of Human Papillomavirus are responsible for 90% of cases of cervical cancer worldwide. Inflammation of squamous cells is mainly linked to HPV. In Gabon, HPV is endemic and circulates among the female population. The study aimed to determine the prevalence of HR-HPV genotypes and to investigate the correlation between squamous cell inflammation and HPV viral load in infected women in Gabon.

METHODS

The cross-sectional study was conducted at Libreville University Hospital Center (UHC) and National Public Health Laboratory from March to May 2024 among 399 women. Two cervical smears were taken. Genotype detection was carried out by multiplex fluorescence real-time PCR in the NPHL virology unit. Cytology was carried out in UHC's anatomic-pathology laboratory. Data were analyzed by SPSS software. Graphs were plotted using Microsoft Excel 2016.

RESULTS

The prevalence of Human Papillomavirus was 26.1% (95% CI: 22-30.6). The prevalence of HR-HPV genotypes was 24.8%. The most common HR-HPV genotypes were HPV-16/52/18/35/56/58/53/68. The rate of multiple HPV infections was 29.8% and 95.2% for the HR-HPV infection rate. Viral load was significantly correlated with squamous cell inflammation (r = 0.977 and P = 0.001).

CONCLUSION

HR-HPV infection remains a concern in women, however early screening is necessary for optimal monitoring and management. HR-HPV viral load is a predictive marker of squamous cell inflammation.

摘要

背景

高危型人乳头瘤病毒（HPV）基因型占全球宫颈癌病例的 90%。鳞状细胞炎症主要与 HPV 相关。在加蓬，HPV 呈地方性流行，并在女性人群中传播。本研究旨在确定高危型 HPV 基因型的流行率，并探讨感染 HPV 的女性中鳞状细胞炎症与 HPV 病毒载量之间的相关性。

方法

2024 年 3 月至 5 月，在利伯维尔大学医院中心（UHC）和国家公共卫生实验室进行了一项横断面研究，共纳入 399 名女性。采集两份宫颈涂片。在 NPHL 病毒学实验室采用多重荧光实时 PCR 检测基因型。细胞学检查在 UHC 的解剖病理学实验室进行。数据分析采用 SPSS 软件。图表使用 Microsoft Excel 2016 绘制。

结果

人乳头瘤病毒的流行率为 26.1%（95%CI：22-30.6）。高危型 HPV 基因型的流行率为 24.8%。最常见的高危型 HPV 基因型为 HPV-16/52/18/35/56/58/53/68。多重 HPV 感染率为 29.8%，高危型 HPV 感染率为 95.2%。病毒载量与鳞状细胞炎症呈显著相关（r=0.977，P=0.001）。