Lan Hujiao, Zhao Yating, Huang Minjun, Wang Wenxu, Liu Jianzhong
College of Life Sciences, Zhejiang Normal University, Jinhua, China.
Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, Zhejiang Normal University, Jinhua, China.
Autophagy. 2025 Jan;21(1):246-248. doi: 10.1080/15548627.2024.2414451. Epub 2024 Oct 16.
Extensive interconnection has been established between clathrin-mediated endocytosis (CME) and the macroautophagy/autophagy pathway in yeast and mammals. However, the evidence that connects these two pathways in plants has been limited. Starting from the phenotypic similarities in carbon starvation and immune responses shared between the double mutant of CLC2 (clathrin light chain 2) and , , and the mutant in Arabidopsis, we found that the autophagy pathway is compromised in the mutant. Subsequently, we demonstrated that CLC2 interacts specifically with ATG8h and ATG8i, two clade II ATG8 isoforms. The CLC2-ATG8h/ATG8i interaction depends on an Atg8-family interacting motif (AIM) present in CLC2 and an AIMs docking site (ADS) present in ATG8h, respectively. In addition, CLC2-GFP is subjected to autophagic degradation and the degradation of GFP-ATG8h is significantly reduced in the mutant. Last, simultaneously knocking out and enhances disease resistance, corroborating the functional relevance of the CLC2-ATG8h/8i interactions. These findings reveal that CME and the autophagy pathway are intersected via CLC2-ATG8h/8i interactions in Arabidopsis.: ADS, AIMs docking site; AIM, Atg8-family interacting motif; ATG, autophagy related; CLC, CLATHRIN LIGHT CHAIN; CME, clathrin-mediated endocytosis; RBOHD, RESPIRATORY BURST OXIDASE HOMOLOGUE PROTEIN D; ROS, reactive oxygen species; PM, plasma membrane; SA, salicylic acid.
在酵母和哺乳动物中，网格蛋白介导的内吞作用（CME）与巨自噬/自噬途径之间已建立了广泛的相互联系。然而，在植物中连接这两条途径的证据一直有限。从拟南芥中CLC2（网格蛋白轻链2）双突变体与 、 以及 突变体在碳饥饿和免疫反应方面的表型相似性出发，我们发现 突变体中的自噬途径受损。随后，我们证明CLC2与两种II类ATG8亚型ATG8h和ATG8i特异性相互作用。CLC2 - ATG8h/ATG8i相互作用分别依赖于CLC2中存在的Atg8家族相互作用基序（AIM）和ATG8h中存在的AIM对接位点（ADS）。此外，CLC2 - GFP会经历自噬降解，而在 突变体中GFP - ATG8h的降解显著减少。最后，同时敲除 和 可增强抗病性，证实了CLC2 - ATG8h/8i相互作用的功能相关性。这些发现揭示了在拟南芥中CME和自噬途径通过CLC2 - ATG8h/8i相互作用相交：ADS，AIM对接位点；AIM，Atg8家族相互作用基序；ATG，自噬相关；CLC，网格蛋白轻链；CME，网格蛋白介导的内吞作用；RBOHD，呼吸爆发氧化酶同源蛋白D；ROS，活性氧；PM，质膜；SA，水杨酸。
