College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing, 102617, China; Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, Beijing, 102617, China.
College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing, 102617, China; Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, Beijing, 102617, China.
Anal Chim Acta. 2024 Nov 15;1329:343263. doi: 10.1016/j.aca.2024.343263. Epub 2024 Sep 19.
N-acetylneuraminic acid (NANA) can be used as a biomarker for many types of cancers. Currently, there are various methods for detecting NANA but showing some shortcomings that limit the real-time diagnosis of cancer. In contrast, fluorescence analysis has obvious advantages such as low cost, fast response time, and easy operation, and it also enables visual detection for real-time cancer monitoring. Therefore, the establishment of an efficient and rapid detection method is essential for the early prevention and treatment of the disease. Based on the properties of layered rare-earth hydroxide (LRH), we synthesized a dual-emission fluorescent material (NDC/SDS-LEuH), and further fabricated a fluorescent nanoprobe (ANP) for the detection of NANA. The probe has the advantages of high sensitivity (LOD = 32.9 μM) and high selectivity with fast response. During the sensing process, the dual emission of the probe shows opposite changes due to the photoinduced electron transfer (PET) effect and the interaction between NANA and the probe. The color changes of the system can be observed under UV irradiation. Therefore, a visual platform was developed to detect NANA with a LOD of 0.09 mM. In addition, a probe hydrogel was prepared, which can be applied in the anti-counterfeiting to improve the difficulty of counterfeiting and the security of anti-counterfeiting. The probe achieves ratiometric fluorescence detection of NANA, which reduces background interference and improves the accuracy of detection. A visual detection platform was fabricated to realize the real-time detection. In addition, the prepared probe hydrogel showed the potential applications in anti-counterfeiting, which provided new ideas for the design and application of anti-counterfeiting materials.
N-乙酰神经氨酸（NANA）可用作许多类型癌症的生物标志物。目前，有多种检测 NANA 的方法，但存在一些限制癌症实时诊断的缺点。相比之下，荧光分析具有成本低、响应时间快、操作简单等明显优势，还能实现实时癌症监测的可视化检测。因此，建立一种高效、快速的检测方法对于疾病的早期预防和治疗至关重要。基于层状稀土氢氧化物（LRH）的性质，我们合成了一种双发射荧光材料（NDC/SDS-LEuH），并进一步制备了用于检测 NANA 的荧光纳米探针（ANP）。该探针具有高灵敏度（LOD=32.9 μM）和高选择性，响应速度快。在传感过程中，由于光诱导电子转移（PET）效应和 NANA 与探针之间的相互作用，探针的双发射表现出相反的变化。在紫外光照射下可以观察到体系的颜色变化。因此，开发了一种可视化平台，用于检测 NANA，其 LOD 为 0.09 mM。此外，还制备了探针水凝胶，可用于防伪，提高防伪难度和安全性。探针实现了 NANA 的比率荧光检测，减少了背景干扰，提高了检测的准确性。构建了可视化检测平台，实现了实时检测。此外，所制备的探针水凝胶在防伪方面具有潜在应用，为防伪材料的设计和应用提供了新的思路。
