Temperature governs the community assembly of root-associated ectomycorrhizal fungi in alpine forests on the Qinghai-Tibetan Plateau.

Unraveling the assembly processes of ectomycorrhizal (ECM) fungal communities in changing environments is crucial for forecasting the impacts of climate change on forests. However, the assembly processes and key drivers of root-associated ECM fungal communities in alpine coniferous forests remain poorly understood. To address this knowledge gap, we conducted sampling in 65 monodominant alpine coniferous forests, which encompass 11 plant species belonging to three genera (Abies, Pinus, and Picea) within the Pinaceae family, all located on the Qinghai-Tibetan Plateau. We employed a combination of null model and multivariate analyses to elucidate the drivers and assembly processes of ECM fungal communities. Our results revealed significant variation in the composition and diversity of root-associated ECM fungal communities among Abies, Pinus, and Picea, indicating specific preferences for ECM fungi among Pinaceae genera. Importantly, mean annual temperature (MAT) emerged as the primary driver of these variations and regulated the assembly processes within the community of root-associated ECM fungi. As MAT temperature, the α-diversity of these fungi significantly decreased, suggesting that increased temperature may reduce the species diversity of root-associated ECM fungi in alpine forests. Furthermore, stochastic processes, such as dispersal limitation and drift, became more influential as MAT increased. Conversely, the role of deterministic processes, particularly heterogeneous selection, in shaping the ECM fungal community assembly weakened with increasing MAT. This study provides novel theoretical insights into the processes of ECM fungal community assembly in alpine forests, emphasizing the pivotal role of temperature in regulating the assembly processes and compositional dynamics of root-associated ECM fungal communities in these unique environments.