Chinese PLA Medical School, Chinese PLA General Hospital, Beijing 100853, China.
State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem, Senior Department of Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071, China.
Zool Res. 2024 Nov 18;45(6):1276-1286. doi: 10.24272/j.issn.2095-8137.2024.173.
Hematopoiesis originates in the yolk sac, which forms prior to the establishment of blood circulation and exhibits distinct developmental processes between primates and mice. Despite increasing appreciation of yolk sac hematopoiesis for its lifelong contribution to the adult hematopoietic system and its regulatory roles in organogenesis, cross-species differences, particularly before the onset of blood circulation, remain incompletely understood. In this study, we constructed an integrative cross-species transcriptome atlas of pre-circulation hematopoiesis in humans, monkeys ( ), and mice. This analysis identified conserved populations between primates and mice, while also revealing more differentiated myeloid, erythroid, and megakaryocytic lineages in pre-circulation primates compared to mice. Specifically, -expressing macrophages were detected in primates before the onset of blood circulation but were absent in mice. Cell-cell communication analysis identified extraembryonic mesoderm cells as a potential supportive niche for macrophage generation, with ligand-receptor interactions between macrophages and other cell populations in the human yolk sac. Interestingly, pre-circulation macrophages exhibited hallmark signatures reminiscent of a macrophage subset that positively regulates hematopoietic stem cell generation. Our findings provide a valuable cross-species resource, advancing our understanding of human pre-circulation yolk sac hematopoiesis and offering a theoretical basis for the regeneration of functional blood cells.
造血发生于卵黄囊，其形成于血液循环建立之前，并在灵长类动物和小鼠之间表现出明显的发育过程。尽管人们越来越认识到卵黄囊造血对于成年造血系统的终身贡献及其在器官发生中的调节作用，但种间差异，尤其是在血液循环开始之前，仍不完全清楚。在这项研究中，我们构建了人类、猴子（ ）和小鼠的血液循环前造血的综合跨物种转录组图谱。这项分析鉴定了灵长类动物和小鼠之间的保守群体，同时还揭示了与小鼠相比，血液循环前的灵长类动物中更分化的髓样、红系和巨核细胞谱系。具体而言，在血液循环开始之前，在灵长类动物中检测到表达 的巨噬细胞，但在小鼠中不存在。细胞间通讯分析将 胚外中胚层细胞鉴定为巨噬细胞生成的潜在支持龛，人类卵黄囊中巨噬细胞与其他细胞群体之间存在配体-受体相互作用。有趣的是，血液循环前的 巨噬细胞表现出类似于正向调节造血干细胞生成的巨噬细胞亚群的特征标志。我们的研究结果提供了一个有价值的跨物种资源，推进了我们对人类血液循环前卵黄囊造血的理解，并为功能性血细胞的再生提供了理论基础。
