Tang Liqiu, Lv Jinzhen, Zhang Xueying, Wang Chen-Zhu, Wang Dehua
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
School of Life Sciences, Shandong University, Qingdao 266237, China.
J Exp Biol. 2024 Dec 1;227(23). doi: 10.1242/jeb.249264. Epub 2024 Dec 3.
Leptin is a hormone that is secreted by adipocytes and may promote energy expenditure by increasing thermogenesis. Our previous studies have shown that thermo-transient receptor potentials (thermo-TRPs) and gut microbiota are associated with thermoregulation in Mongolian gerbils, which are characterized by relative high serum leptin concentrations. Here, we tested whether leptin can stimulate non-shivering thermogenesis (NST) in Mongolian gerbils, and whether thermo-TRPs and gut microbiota are involved in leptin-induced thermogenesis. First, gerbils were given acute leptin treatment (ALT) with different doses. Results showed that ALT significantly increased the body temperature of gerbils and changed the composition of gut microbiota. Moreover, ALT groups showed a trend towards increased expression of uncoupling protein 1 (UCP1) in brown adipose tissue (BAT). Then, we investigated the effect of chronic leptin treatment (CLT) on gerbils. Surprisingly, CLT did not affect gerbils' food intake and body mass, but it significantly increased the body temperature at the end. Further, CLT did not affect the expression of thermogenic markers in BAT, white adipose tissue (WAT) or skeletal muscle. However, CLT increased the expression of leptin receptors and TRPV2 in the small intestine and affected the composition of gut microbiota. Together, our data suggest leptin may increase body temperature by regulating gut microbiota. In conclusion, serum hyperleptin in Mongolian gerbils is beneficial for adapting to cold environments, and TRPV2 and gut microbiota are involved.
瘦素是一种由脂肪细胞分泌的激素，可能通过增加产热来促进能量消耗。我们之前的研究表明，热瞬态受体电位（thermo-TRPs）和肠道微生物群与蒙古沙鼠的体温调节有关，蒙古沙鼠的特征是血清瘦素浓度相对较高。在此，我们测试了瘦素是否能刺激蒙古沙鼠的非颤抖性产热（NST），以及thermo-TRPs和肠道微生物群是否参与瘦素诱导的产热。首先，给沙鼠进行不同剂量的急性瘦素处理（ALT）。结果显示，ALT显著提高了沙鼠的体温，并改变了肠道微生物群的组成。此外，ALT组棕色脂肪组织（BAT）中解偶联蛋白1（UCP1）的表达有增加趋势。然后，我们研究了慢性瘦素处理（CLT）对沙鼠的影响。令人惊讶的是，CLT不影响沙鼠的食物摄入量和体重，但最终显著提高了体温。此外，CLT不影响BAT、白色脂肪组织（WAT）或骨骼肌中产热标志物的表达。然而，CLT增加了小肠中瘦素受体和TRPV2的表达，并影响了肠道微生物群的组成。总之，我们的数据表明瘦素可能通过调节肠道微生物群来提高体温。综上所述，蒙古沙鼠的血清高瘦素水平有利于适应寒冷环境，TRPV2和肠道微生物群参与其中。
