• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

固定化学计量的TRPC1-TRPC5串联体对G蛋白的独特反应。

Unique responses of the fixed stoichiometric TRPC1-TRPC5 concatemer to G proteins.

作者信息

Kang Hana, So Insuk

机构信息

Department of Physiology and Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.

Institute of Human-Environment Interface Biology, Seoul National University, Seoul, Republic of Korea.

出版信息

Front Physiol. 2024 Sep 27;15:1392980. doi: 10.3389/fphys.2024.1392980. eCollection 2024.

DOI:10.3389/fphys.2024.1392980
PMID:39397856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11466768/
Abstract

Transient receptor potential canonical (TRPC)5 channel is a non-selective cation channel that plays a significant role in membrane depolarization and calcium influx. TRPC5 not only forms homotetramers itself but also heterotetramers with TRPC1. However, accurately testing and confirming these heterotetrameric channels at specific ratios has proven challenging. Therefore, creating heteromeric concatemers of TRPC5 and TRPC1 with a fixed stoichiometry of 1:1 becomes essential. This study aims to meticulously identify and reaffirm the properties of TRPC5 homomers and heteromers with a 1:1 fixed stoichiometry to determine the optimal ratio for the TRPC1/5 heterotetramer. The overall characteristics were consistent with those of the previous studies, but several specific features were different. The TRPC1-TRPC5 concatemer is activated by Englerin A and GQL, whereas carbachol alone does not trigger its activation. Additionally, GQL significantly inhibited the current when co-expressed with the concatemer. Interestingly, carbachol can activate the TRPC1-TRPC5 concatemer in the presence of internal GTPγS, highlighting the influence of intracellular signaling conditions on its activation. Meanwhile, the TRPC5-TRPC5 concatemer is responsive to both carbachol and Englerin A. In conclusion, we provide evidence that the TRPC1-TRPC5 heteromeric concatemer with fixed stoichiometry need specific conditions to respond to carbachol, whereas the TRPC5-TRPC5 homomeric concatemer responds physiologically to carbachol. Additional research may be necessary to ascertain the optimal stoichiometry for the TRPC1-TRPC5 concatemer to enhance its electrophysiological properties.

摘要

瞬时受体电位香草酸亚型5（TRPC）5通道是一种非选择性阳离子通道，在膜去极化和钙内流中起重要作用。TRPC5不仅自身形成同源四聚体，还与TRPC1形成异源四聚体。然而，准确检测和确认这些特定比例的异源四聚体通道已被证明具有挑战性。因此，创建具有1:1固定化学计量比的TRPC5和TRPC1异源连接体变得至关重要。本研究旨在精心鉴定和再次确认具有1:1固定化学计量比的TRPC5同源体和异源体的特性，以确定TRPC1/5异源四聚体的最佳比例。总体特征与先前研究一致，但有几个特定特征有所不同。TRPC1-TRPC5连接体被恩格勒菌素A和GQL激活，而单独的卡巴胆碱不会触发其激活。此外，GQL与连接体共表达时会显著抑制电流。有趣的是，在细胞内存在GTPγS的情况下，卡巴胆碱可以激活TRPC1-TRPC5连接体，突出了细胞内信号传导条件对其激活的影响。同时，TRPC5-TRPC5连接体对卡巴胆碱和恩格勒菌素A均有反应。总之，我们提供的证据表明，具有固定化学计量比的TRPC1-TRPC5异源连接体需要特定条件才能对卡巴胆碱作出反应，而TRPC5-TRPC5同源连接体对卡巴胆碱有生理反应。可能需要进一步研究以确定TRPC1-TRPC5连接体的最佳化学计量比，以增强其电生理特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/7b9d3a46fe74/fphys-15-1392980-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/e7966823ece9/fphys-15-1392980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/7e2532e9bc56/fphys-15-1392980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/ae8a74fe1681/fphys-15-1392980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/b2ed8baaf763/fphys-15-1392980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/9af834ee86e0/fphys-15-1392980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/ad5fb9728ed5/fphys-15-1392980-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/370d13261777/fphys-15-1392980-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/7b9d3a46fe74/fphys-15-1392980-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/e7966823ece9/fphys-15-1392980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/7e2532e9bc56/fphys-15-1392980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/ae8a74fe1681/fphys-15-1392980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/b2ed8baaf763/fphys-15-1392980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/9af834ee86e0/fphys-15-1392980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/ad5fb9728ed5/fphys-15-1392980-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/370d13261777/fphys-15-1392980-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d17/11466768/7b9d3a46fe74/fphys-15-1392980-g008.jpg

相似文献

1
Unique responses of the fixed stoichiometric TRPC1-TRPC5 concatemer to G proteins.固定化学计量的TRPC1-TRPC5串联体对G蛋白的独特反应。
Front Physiol. 2024 Sep 27;15:1392980. doi: 10.3389/fphys.2024.1392980. eCollection 2024.
2
TRPC1 as a negative regulator for TRPC4 and TRPC5 channels.TRPC1 作为 TRPC4 和 TRPC5 通道的负调节剂。
Pflugers Arch. 2019 Aug;471(8):1045-1053. doi: 10.1007/s00424-019-02289-w. Epub 2019 Jun 20.
3
Identification of an (-)-englerin A analogue, which antagonizes (-)-englerin A at TRPC1/4/5 channels.鉴定出一种 (-)-englerin A 类似物，该类似物在 TRPC1/4/5 通道上拮抗 (-)-englerin A。
Br J Pharmacol. 2018 Mar;175(5):830-839. doi: 10.1111/bph.14128. Epub 2018 Jan 25.
4
Tonantzitlolone is a nanomolar potency activator of transient receptor potential canonical 1/4/5 channels.托烷司琼酮是一种纳摩尔效力的瞬时受体电位经典通道 1/4/5 的激活剂。
Br J Pharmacol. 2018 Aug;175(16):3361-3368. doi: 10.1111/bph.14379. Epub 2018 Jun 28.
5
Potent, selective, and subunit-dependent activation of TRPC5 channels by a xanthine derivative.一种黄嘌呤衍生物对 TRPC5 通道的强效、选择性和亚基依赖性激活。
Br J Pharmacol. 2019 Oct;176(20):3924-3938. doi: 10.1111/bph.14791. Epub 2019 Sep 6.
6
Opposite regulatory effects of TRPC1 and TRPC5 on neurite outgrowth in PC12 cells.TRPC1 和 TRPC5 对 PC12 细胞突起生长的相反调节作用。
Cell Signal. 2012 Apr;24(4):899-906. doi: 10.1016/j.cellsig.2011.12.011. Epub 2011 Dec 19.
7
The interaction domains of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heteromultimeric channels.瞬时受体电位经典型（TRPC）1/4和TRPC1/5异源多聚体通道的相互作用结构域。
Biochem Biophys Res Commun. 2016 Jun 3;474(3):476-481. doi: 10.1016/j.bbrc.2016.04.138. Epub 2016 Apr 27.
8
Differential PI(4,5)P sensitivities of TRPC4, C5 homomeric and TRPC1/4, C1/5 heteromeric channels.TRPC4、C5 同源和 TRPC1/4、C1/5 异源通道对 PI(4,5)P 的差异敏感性。
Sci Rep. 2019 Feb 12;9(1):1849. doi: 10.1038/s41598-018-38443-0.
9
Englerin A-sensing charged residues for transient receptor potential canonical 5 channel activation.恩格勒菌素A感知瞬时受体电位香草酸亚型5通道激活的带电残基。
Korean J Physiol Pharmacol. 2019 May;23(3):191-201. doi: 10.4196/kjpp.2019.23.3.191. Epub 2019 Apr 24.
10
TRPC1 proteins confer PKC and phosphoinositol activation on native heteromeric TRPC1/C5 channels in vascular smooth muscle: comparative study of wild-type and TRPC1-/- mice.TRPC1 蛋白使血管平滑肌中原位异源型 TRPC1/C5 通道的 PKC 和磷酸肌醇激活：野生型和 TRPC1-/- 小鼠的比较研究。
FASEB J. 2012 Jan;26(1):409-19. doi: 10.1096/fj.11-185611. Epub 2011 Oct 3.

本文引用的文献

1
A pentameric TRPV3 channel with a dilated pore.一个具有扩张孔道的五聚体 TRPV3 通道。
Nature. 2023 Sep;621(7977):206-214. doi: 10.1038/s41586-023-06470-1. Epub 2023 Aug 30.
2
Pore residues of transient receptor potential channels canonical 1 and 4 heteromer determine channel properties.瞬时受体电位通道经典型 1 和 4 异源二聚体的孔残留物决定了通道特性。
Am J Physiol Cell Physiol. 2023 Jul 1;325(1):C42-C51. doi: 10.1152/ajpcell.00488.2022. Epub 2023 May 22.
3
Molecular architecture of the Gα-bound TRPC5 ion channel.Gα 结合型 TRPC5 离子通道的分子结构。
Nat Commun. 2023 May 3;14(1):2550. doi: 10.1038/s41467-023-38281-3.
4
Subunit composition, molecular environment, and activation of native TRPC channels encoded by their interactomes.由其相互作用组编码的天然瞬时受体电位阳离子通道（TRPC通道）的亚基组成、分子环境及激活
Neuron. 2022 Dec 21;110(24):4162-4175.e7. doi: 10.1016/j.neuron.2022.09.029. Epub 2022 Oct 17.
5
Lower troponin expression in the right ventricle of rats explains interventricular differences in E-C coupling.大鼠右心室心尖球蛋白表达降低解释了室间电耦合的差异。
J Gen Physiol. 2022 Mar 7;154(3). doi: 10.1085/jgp.202112949. Epub 2022 Jan 31.
6
Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.人类 TRPC5 通道受两种不同抑制剂抑制的结构基础。
Elife. 2021 Mar 8;10:e63429. doi: 10.7554/eLife.63429.
7
Structural basis of TRPC4 regulation by calmodulin and pharmacological agents.钙调蛋白和药物调节 TRPC4 的结构基础。
Elife. 2020 Nov 25;9:e60603. doi: 10.7554/eLife.60603.
8
Human TRPC5 structures reveal interaction of a xanthine-based TRPC1/4/5 inhibitor with a conserved lipid binding site.人源 TRPC5 结构揭示黄嘌呤类 TRPC1/4/5 抑制剂与保守脂质结合位点的相互作用。
Commun Biol. 2020 Nov 23;3(1):704. doi: 10.1038/s42003-020-01437-8.
9
Thermosensitivity of the voltage-dependent activation of calcium homeostasis modulator 1 (calhm1) ion channel.钙稳态调节蛋白 1（calhm1）离子通道电压依赖性激活的温度敏感性。
Biochem Biophys Res Commun. 2021 Jan 1;534:590-596. doi: 10.1016/j.bbrc.2020.11.035. Epub 2020 Nov 14.
10
TRPC1/4/5 channels contribute to morphine-induced analgesic tolerance and hyperalgesia by enhancing spinal synaptic potentiation and structural plasticity.TRPC1/4/5 通道通过增强脊髓突触增强和结构可塑性，导致吗啡诱导的镇痛耐受和痛觉过敏。
FASEB J. 2020 Jun;34(6):8526-8543. doi: 10.1096/fj.202000154RR. Epub 2020 May 2.