甘草次酸通过降低肺炎球菌溶血素的毒性来减轻肺炎球菌感染引起的肺部炎症。
Glycyrrhetinic acid reduces lung inflammation caused by pneumococcal infection by reducing the toxicity of pneumolysin.
作者信息
Xu Yan, Ding Ying, Wu Hongji, Li Donglin, Li Yudi, Hu Yibo, Meng Haoji
机构信息
Department of Pediatrics, The First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, Henan, 450000, China.
School of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, 450000, China.
出版信息
Heliyon. 2024 Sep 29;10(19):e38611. doi: 10.1016/j.heliyon.2024.e38611. eCollection 2024 Oct 15.
OBJECTIVE
In this study, to provide new methods for the treatment of infection, we aimed to describe the anti-inflammatory and antibacterial value of glycyrrhetinic acid on the basis of its inhibitory effect on bacterial growth (without killing the bacteria) and its reduction of the toxicity of
METHODS
A mouse model was established via intranasal administration of D39, and glycyrrhetinic acid was subcutaneously injected for treatment. The wet‒dry ratio, bacterial flora content and inflammatory factor levels in the mouse lungs were determined. Cell experiments were used to evaluate glycyrrhetinic acid-mediated inhibition of PLY hemolysis and A549 cell death, and WB was used to measure glycyrrhetinic acid-mediated inhibition of PLY oligomerization.
RESULTS
Glycyrrhetinic acid reduced the levels of inflammatory factors, the dry‒wet ratio, the abundance of in the lungs of infected mice, pneumolysin-mediated A549 cell death, erythrocyte hemolysis and PLY oligoplasia.
CONCLUSION
Glycyrrhetinic acid can reduce the virulence of by preventing the oligomerization of PLY.
目的
在本研究中，为感染治疗提供新方法，我们旨在基于甘草次酸对细菌生长的抑制作用（不杀死细菌）及其对[毒性名称缺失]毒性的降低，描述其抗炎和抗菌价值。
方法
通过鼻内给予D39建立小鼠模型，并皮下注射甘草次酸进行治疗。测定小鼠肺中的湿干比、细菌菌群含量和炎症因子水平。采用细胞实验评估甘草次酸介导的对PLY溶血和A549细胞死亡的抑制作用，并使用WB测量甘草次酸介导的对PLY寡聚化的抑制作用。
结果
甘草次酸降低了感染小鼠肺中的炎症因子水平、湿干比、[细菌名称缺失]丰度、肺炎溶血素介导的A549细胞死亡、红细胞溶血和PLY寡聚化。
结论
甘草次酸可通过阻止PLY的寡聚化来降低[细菌名称缺失]的毒力。
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