Owrang Mina, Gholami Alamara
Faculty of Medicine, Sari branch, Islamic Azad University, Sari, Iran.
Department of Biological Sciences and Technologies, Faculty of Basic Science, Islamic Azad University, Sari branch, Sari, Iran.
Heliyon. 2024 Sep 24;10(19):e38395. doi: 10.1016/j.heliyon.2024.e38395. eCollection 2024 Oct 15.
The global challenge to human health is significantly heightened by the resistance of harmful bacteria to antimicrobial treatments. Given the limited advancement in developing new antimicrobial medications, exploring innovative strategies is imperative to tackle the challenge of resistance to multiple drugs. Furthermore, there is a growing emphasis on the environmentally friendly synthesis of nanoparticles with potent medicinal attributes, specifically those targeting virulence, to combat the rise of multidrug resistance. Focusing on the inhibition of virulence factors and biofilms influenced by quorum sensing has become a promising and novel strategy in the development of anti-infective drugs. An aqueous extract of leaves was used to create green-synthesized silver nanoparticles, or AgNPs. X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and UV-visible absorption spectroscopy were used to characterize the AgNPs. The impact of AgNPs on the virulence factors and biofilms of PAO1, mediated by quorum sensing, was assessed at concentrations below the minimum inhibitory concentration (sub-MIC). Sub-MIC concentrations of Green-synthesized AgNPs inhibited various . virulence factors, including bacterial motility (89 % inhibition), pyocyanin production (81.48 % inhibition), pyoverdin production (55.80 % inhibition), elastase activity (87.43 % inhibition), exoprotease activity (75.60 % inhibition), and rhamnolipid production (71.28 % inhibition). Additionally, these AgNPs demonstrated 80 % inhibition of biofilms. The in vitro efficacy of green-synthesized AgNPs against can be utilized for the creation of alternative therapeutic agents for managing bacterial infections, particularly for topical application in cases such as wound infections. Additionally, they can be used for surface coating to inhibit the attachment of bacteria to medical devices.
有害细菌对抗菌治疗的耐药性显著加剧了对人类健康的全球挑战。鉴于开发新型抗菌药物的进展有限，探索创新策略对于应对多重耐药性挑战至关重要。此外，越来越强调以环境友好的方式合成具有强大药用特性的纳米颗粒，特别是那些针对毒力的纳米颗粒，以对抗多重耐药性的上升。关注群体感应影响的毒力因子和生物膜的抑制已成为抗感染药物开发中一种有前景的新策略。叶片的水提取物被用于制备绿色合成银纳米颗粒（AgNPs）。采用X射线衍射（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和紫外可见吸收光谱对AgNPs进行表征。在低于最低抑菌浓度（亚MIC）的浓度下，评估了AgNPs对群体感应介导的PAO1毒力因子和生物膜的影响。绿色合成的AgNPs在亚MIC浓度下抑制了多种毒力因子，包括细菌运动性（抑制率89%）、绿脓菌素产生（抑制率81.48%）、绿脓菌素产生（抑制率55.80%）、弹性蛋白酶活性（抑制率87.43%）、外蛋白酶活性（抑制率75.60%）和鼠李糖脂产生（抑制率71.28%）。此外，这些AgNPs对生物膜的抑制率为80%。绿色合成的AgNPs的体外疗效可用于开发治疗细菌感染的替代治疗剂，特别是用于伤口感染等局部应用。此外，它们可用于表面涂层，以抑制细菌附着在医疗设备上。
