Huang Rongzheng, Zhang Fanfan, Wang Xuzhe, Yang Fan, Ma Chunhui
Grassland Science, School of Animal Technology, Shihezi University, Shihezi 832000, China.
ACS Omega. 2024 Sep 23;9(40):41148-41156. doi: 10.1021/acsomega.3c08947. eCollection 2024 Oct 8.
The antibacterial mechanism of condensed tannins (CTs) obtained from tea has been elucidated, but the mechanism of legume-derived CTs remains unclear. The mechanisms of legume- and tea-derived CTs probably differ due to the diverse compositions of CTs. Previous research found that sainfoin CTs directly inhibited the growth of . The present study investigated the inhibition mechanism of CTs against SF11 (SF11) through proteomic analysis. The results showed that the minimum inhibitory concentration (MIC) of CTs against SF11 was 1500 mg/L and that CTs increased cell membrane permeability in a dose-dependent manner. In total, 418 differentially expressed proteins (DEPs) were identified between the CT treatment and the control, among which 341 were down-regulated and 77 were up-regulated in the CT treatment. The protein interaction network showed that the expression of only two DEPs was highly different between CT treated and control (|log2FC|> 2); the D protein was up-regulated in the CT-treated group, which was involved in ATP synthesis; down-regulated DEPs were most involved in lipoteichoic acid synthesis, peptidoglycan synthesis, and glycine metabolism. Twenty-seven proteins were not detected after CT treatment, which were involved in functions including fatty acid synthesis, RNA synthesis and translation, drug resistance, and cell membrane permeability in SF11. Therefore, the findings suggest that the inhibition mechanism of CTs may be related to cell membrane damage and inhibition of cell reproduction.
从茶叶中提取的缩合单宁（CTs）的抗菌机制已得到阐明，但豆类来源的CTs的作用机制仍不清楚。由于CTs的组成不同，豆类和茶叶来源的CTs的作用机制可能存在差异。先前的研究发现红豆草CTs可直接抑制……的生长。本研究通过蛋白质组学分析探讨了CTs对SF11（SF11）的抑制机制。结果表明，CTs对SF11的最小抑菌浓度（MIC）为1500mg/L，且CTs以剂量依赖的方式增加细胞膜通透性。共鉴定出CT处理组与对照组之间有418个差异表达蛋白（DEP），其中CT处理组中有341个下调，77个上调。蛋白质相互作用网络显示，CT处理组与对照组之间只有两个DEP的表达差异很大（|log2FC|>2）；D蛋白在CT处理组中上调，参与ATP合成；下调的DEP大多参与脂磷壁酸合成、肽聚糖合成和甘氨酸代谢。CT处理后有27种蛋白质未被检测到，这些蛋白质参与了SF11中的脂肪酸合成、RNA合成与翻译、耐药性和细胞膜通透性等功能。因此，研究结果表明CTs的抑制机制可能与细胞膜损伤和细胞繁殖抑制有关。
