Mitchell W J
Department of Brewing and Biological Sciences, Heriot-Watt University, Edinburgh, UK.
Microbiol Sci. 1985 Nov;2(11):330-4, 339.
Despite the fact that bacterial solute transport has been intensively studied for the last two decades, it has not yet been possible to describe the molecular mechanism of any transport process, largely due to the difficulties involved in handling membrane proteins and the lack of convenient in vitro assay procedures. The phosphoenolpyruvate: glycose phosphotransferase, a multiprotein system consisting of both soluble and membrane-bound components and with an associated chemical reaction which can be measured in cell-free extracts, has considerable potential for attaining this goal. Although the PTS is extremely complex, our understanding of the mechanisms by which it mediates and regulates carbohydrate transport in enteric bacteria is at an advanced stage.
尽管在过去二十年中对细菌溶质转运进行了深入研究，但由于处理膜蛋白存在困难且缺乏便捷的体外测定程序，尚未能够描述任何转运过程的分子机制。磷酸烯醇丙酮酸：葡萄糖磷酸转移酶是一种由可溶性和膜结合成分组成的多蛋白系统，其相关化学反应可在无细胞提取物中测量，在实现这一目标方面具有相当大的潜力。尽管磷酸转移酶系统极其复杂，但我们对其介导和调节肠道细菌中碳水化合物转运机制的理解已处于先进阶段。
