Department of Medical Oncology, Nanyang Central Hospital, Nanyang, 473000, China.
Epigenomics. 2024;16(19-20):1287-1299. doi: 10.1080/17501911.2024.2410697. Epub 2024 Oct 14.
To explore precise function and underlying mechanism of circ_0006988 in gastric cancer (GC). GC tissues were collected clinically, and GC cells were purchased from the company. Quantitative real-time polymerase chain reaction and western blot were used to detect mRNA and protein expression. Functional analysis was performed through CCK-8, Transwell and scratch experiment. Binding relationship was validated through dual luciferase reporter and RNA immunoprecipitation assays. HGC-27 cells were subcutaneously injected into mice to construct a xenograft tumor model. In GC tissues and cells, circ_0006988 overexpressed, promoting proliferation, migration and invasion. MiRNA-92a-2-5p downregulation or overexpression weakened effects of circ_0006988 silencing on GC progression. circ_0006988 facilitates GC development through miRNA-92a-2-5p/TFAP4 axis.
为了探索 circ_0006988 在胃癌（GC）中的精确功能和潜在机制。临床收集 GC 组织，并从公司购买 GC 细胞。采用实时定量聚合酶链反应和 Western blot 检测 mRNA 和蛋白表达。通过 CCK-8、Transwell 和划痕实验进行功能分析。通过双荧光素酶报告和 RNA 免疫沉淀实验验证结合关系。将 HGC-27 细胞皮下注射到小鼠中构建异种移植肿瘤模型。在 GC 组织和细胞中，circ_0006988 过表达，促进增殖、迁移和侵袭。miRNA-92a-2-5p 下调或过表达减弱了 circ_0006988 沉默对 GC 进展的影响。circ_0006988 通过 miRNA-92a-2-5p/TFAP4 轴促进 GC 的发展。
