Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Colorectal Surgical Unit, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Sci Rep. 2023 Aug 16;13(1):13308. doi: 10.1038/s41598-023-40358-4.
Colorectal cancer (CRC) poses a significant burden on both the healthcare systems as well as individuals. The high mortality rate of CRC may be attributed to its metastatic potential, heterogeneity, and delayed diagnosis. CircRNAs are an essential class of regulatory RNAs that play significant roles in cancers. This study aimed to detect the expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with CRC. This study included 50 CRC patients, 30 individuals with colorectal diseases (non-cancer), and 20 healthy volunteers. By using real-time PCR, the relative expression of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 was determined in the collected blood samples. In addition, ECLIA was used to quantify carcinoembryonic antigen (CEA) level. All circRNAs expression and CEA levels were significantly up-regulated in cancer patients (CRC, colon, rectum) as compared to healthy controls, except circ-SMARCA5. Moreover, there was a significant up-regulation of circRNAs in most non-cancer patients (UC, polyp, piles). Insignificant upregulation was observed in circRNAs and CEA when comparing cancer with non-cancer patients. No correlations were found between the studied parameters and most clinicopathological characteristics of cancer and non-cancer patients. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 were differentially expressed in patients with CRC as well as in non-cancer patients. Circ-SMARCA5 and circ-NOL10 may act as tumor suppressors, while circ-LDLRAD3 and circ-RHOT1 may be oncogenes. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 could be promising markers for the early detection of CRC.
结直肠癌（CRC）给医疗保健系统和个人都带来了巨大的负担。CRC 的高死亡率可能归因于其转移潜力、异质性和诊断延迟。circRNAs 是一类重要的调节 RNA，在癌症中发挥着重要作用。本研究旨在检测 CRC 患者中 circ-SMARCA5、circ-NOL10、circ-LDLRAD3 和 circ-RHOT1 的表达状态。本研究纳入了 50 例 CRC 患者、30 例结直肠疾病（非癌症）患者和 20 名健康志愿者。通过实时 PCR 测定收集的血液样本中 circ-SMARCA5、circ-NOL10、circ-LDLRAD3 和 circ-RHOT1 的相对表达。此外，还使用 ECLIA 定量检测癌胚抗原（CEA）水平。与健康对照组相比，所有 circRNAs 的表达和 CEA 水平在癌症患者（CRC、结肠、直肠）中均显著上调，除 circ-SMARCA5 外。此外，大多数非癌症患者（UC、息肉、痔疮）中 circRNAs 的表达也显著上调。癌症与非癌症患者之间的 circRNAs 和 CEA 上调不明显。研究参数与癌症和非癌症患者的大多数临床病理特征之间未发现相关性。circ-SMARCA5、circ-NOL10、circ-LDLRAD3 和 circ-RHOT1 在 CRC 患者以及非癌症患者中表达不同。circ-SMARCA5 和 circ-NOL10 可能作为肿瘤抑制因子发挥作用，而 circ-LDLRAD3 和 circ-RHOT1 可能是癌基因。circ-SMARCA5、circ-NOL10、circ-LDLRAD3 和 circ-RHOT1 可能是 CRC 早期检测的有前途的标志物。
