Center for Research and Practice in Biotechnology and Genetic Engineering, Istanbul University, Istanbul, Fatih, Turkey.
Molecular Microbiology Unit, Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Kötekli-Muğla, Turkey.
Curr Microbiol. 2024 Oct 14;81(12):404. doi: 10.1007/s00284-024-03908-1.
Rhizobacteria have the ability to compete with pathogenic microorganisms and contribute to plant immunity and defense mechanisms. Their growth and survival in the rhizosphere ensure a biological balance in favor of the host plant. The differential gene expression profiles of B. safensis (MM19) revealed significantly increased expression of prominent genes related to thiamine biosynthesis involving various metabolites and enzymes that participate in the suppression of mycelium growth and pathogen inhibition. Correspondingly, the expression of three major genes (HOG1, FUS3, SGI) involved in the virulence of P. viticola was assessed using qPCR analysis. HOG1 was the highest expressed gene in the pathogen when it was co-cultivated with MM19. Based on these findings, we performed molecular docking and dynamics analysis to explore the interaction between HOG1 and thiamine, as well as expression network analysis constructed using Cytoscape. The functional genomic data related to thiamine biosynthesis and the corresponding pathways ensure a priming role in the antagonistic behavior of B. safensis (MM19) against P. viticola as a support for plant immunity.
根际细菌具有与病原微生物竞争的能力，并有助于植物的免疫和防御机制。它们在根际中的生长和存活确保了有利于宿主植物的生物平衡。B. safensis (MM19) 的差异基因表达谱显示，与硫胺素生物合成相关的显著上调表达的突出基因数量增加，涉及各种参与抑制菌丝生长和病原体抑制的代谢物和酶。相应地，使用 qPCR 分析评估了与 P. viticola 毒力相关的三个主要基因（HOG1、FUS3、SGI）的表达。当与 MM19 共培养时，HOG1 是病原体中表达最高的基因。基于这些发现，我们进行了分子对接和动力学分析，以探索 HOG1 与硫胺素之间的相互作用，以及使用 Cytoscape 构建的表达网络分析。与硫胺素生物合成和相应途径相关的功能基因组数据确保了 B. safensis (MM19) 对 P. viticola 的拮抗行为具有启动作用，作为对植物免疫的支持。
