Jandová A, Mejsnarová B, Motycka K, Coupek J, Sanitrák J, Heyberger K, Pezlarová J
Neoplasma. 1979;26(3):261-6.
Immunochemically active fractions were obtained using Separon Hema-300-glc(R) from serum of lactic dehydrogenase virus (LDV) infected mice and from homogenates of human tumors. The mixture of proteins of tumorous origin from the cytosol giving a positive reaction in the leukocyte adherence inhibition (LAI) test was found in the same fractions showing maximum of absorbance at 340 nm in the spectrophotometer and a corresponding peak in the refractometer. Analogous peaks were not proved in material obtained from healthy controls, but they were found in some human placentas and fetal organs. The LDV fraction obtained from mouse serum served as a "control" antigen in LAI test for human tumor testing, and results corresponded with those obtained using cytosol specific for the tumor under study.
使用Separon Hema - 300 - glc(R)从感染乳酸脱氢酶病毒（LDV）的小鼠血清和人类肿瘤匀浆中获得免疫化学活性组分。在白细胞黏附抑制（LAI）试验中呈阳性反应的来自肿瘤细胞质溶胶的蛋白质混合物，在分光光度计中于340 nm处显示最大吸光度的相同组分中被发现，并且在折射仪中出现相应的峰值。在从健康对照获得的材料中未证实存在类似的峰值，但在一些人类胎盘和胎儿器官中发现了这些峰值。从小鼠血清中获得的LDV组分在用于人类肿瘤检测的LAI试验中用作“对照”抗原，其结果与使用所研究肿瘤的特异性细胞质溶胶获得的结果一致。
