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感染一种能提高L-乳酸:NAD氧化还原酶活性的病毒的小鼠血清的凝胶色谱分析——分离病毒活性和酶活性的尝试。

Gel chromatography of serum from mice infected with a virus elevating L-lactate: NAD oxidoreductase activity--an attempt to separate viral and enzymatic activities.

作者信息

Motycka K, Jandová A, Kriváková M, Coupek J, Pezlarová J

出版信息

Acta Virol. 1976 Feb;20(1):53-60.

PMID:7941
Abstract

Gel chromatography on Spheron P-500 of mouse serum yielded fractions elevating lactate dehydrogenase activity. The order of elution was viral activity, proteins and lactate dehydrogenase activity. Viral contamination occurred also in certain fractions subjected to repeated gel chromatography in which no proteins were detectable.

摘要

对小鼠血清进行Spheron P - 500凝胶色谱分析得到了能提高乳酸脱氢酶活性的组分。洗脱顺序为病毒活性、蛋白质和乳酸脱氢酶活性。在某些经反复凝胶色谱分析且未检测到蛋白质的组分中也出现了病毒污染。

相似文献

1
Gel chromatography of serum from mice infected with a virus elevating L-lactate: NAD oxidoreductase activity--an attempt to separate viral and enzymatic activities.感染一种能提高L-乳酸:NAD氧化还原酶活性的病毒的小鼠血清的凝胶色谱分析——分离病毒活性和酶活性的尝试。
Acta Virol. 1976 Feb;20(1):53-60.
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Persistence and other characteristics of the lactate dehydrogenase-elevating virus (LDH-virus).乳酸脱氢酶升高病毒(LDH病毒)的持续性及其他特性。
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Correlation of extracts obtained by high efficiency gel chromatography of lactic dehydrogenase virus infected mouse serum and cytosol from human tumors using leukocyte adherence inhibition assay.利用白细胞黏附抑制试验对乳酸脱氢酶病毒感染小鼠血清及人肿瘤细胞溶质进行高效凝胶色谱法提取所得提取物的相关性研究。
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Delayed increase in plasma lactic dehydrogenase activity in mouse hepatitis virus-infected mice subsequently infected with lactic dehydrogenase virus.感染乳酸脱氢酶病毒后,小鼠肝炎病毒感染小鼠的血浆乳酸脱氢酶活性延迟增加。
Lab Anim Sci. 1987 Dec;37(6):792-4.
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Mouse strain-specific central nervous system lesions associated with lactate dehydrogenase-elevating virus infection.与乳酸脱氢酶升高病毒感染相关的小鼠品系特异性中枢神经系统病变。
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Detection of lactate dehydrogenase-elevating virus by use of a fluorogenic nuclease reverse transcriptase-polymerase chain reaction assay.使用荧光核酸酶逆转录聚合酶链反应检测乳酸脱氢酶升高病毒。
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False negative results using RT-PCR for detection of lactate dehydrogenase-elevating virus in a tumor cell line.使用逆转录聚合酶链反应(RT-PCR)检测肿瘤细胞系中乳酸脱氢酶升高病毒时的假阴性结果。
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Reduction of plasma clearance rates and immune response by negative charges: lactic dehydrogenase isoenzymes in normal and LDV-infected mice.负电荷对血浆清除率和免疫反应的降低作用:正常及感染乳酸脱氢酶病毒小鼠体内的乳酸脱氢酶同工酶
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The effect of three bleeding techniques on lactic dehydrogenase levels in mice: implications for lactic dehydrogenase virus bioassay.三种出血技术对小鼠乳酸脱氢酶水平的影响:对乳酸脱氢酶病毒生物测定的意义。
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Lactic dehydrogenase virus.乳酸脱氢酶病毒
Virol Monogr. 1975(13):1-121. doi: 10.1007/978-3-7091-8378-6_1.

引用本文的文献

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Potential detrimental effects of rodent viral infections on long-term experiments.啮齿动物病毒感染对长期实验的潜在有害影响。
Vet Res Commun. 1988;12(2-3):199-217. doi: 10.1007/BF00362802.
2
[Fractions of human neoplasms and mice-LDH virus as markers of human cancer].[人类肿瘤的分数及小鼠乳酸脱氢酶病毒作为人类癌症的标志物]
Arch Gynecol Obstet. 1989;245(1-4):476-8. doi: 10.1007/BF02417381.