Cai Jinhong, Guan Shenghong, Hu Xueli, Chen Xuezhao, Liu Xiaosun, Li Shouxin, Tian Jingkui, Wang Ping, Gu Hua, Zhang Xiaoyong
Zhejiang University of Technology, College of Pharmaceutical Science, Hangzhou 310032, China.
Chinese Academy of Sciences, Key Laboratory for Molecular Medicine and Chinese Medicine Preparations, Hangzhou Institute of Medicine (HIM), Hangzhou 310020, China.
J AOAC Int. 2025 Jan 1;108(1):62-77. doi: 10.1093/jaoacint/qsae079.
Premna microphylla Turcz. (PMT) is a traditional food and medicinal plant, which has been used to treat cure hemostasis, rheumatism, and dysentery. However, there is still a lack of a clear understanding of the chemical profile of PMT and its metabolites in vivo.
To establish a rapid and efficient analytical method for the identification of phytochemicals in PMT and their metabolites in vivo.
First, the fingerprint of PMT was established by HPLC with method validation. Then, the phytochemical composition of PMT leaves was identified using ultra-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). Finally, the prototype and correlated metabolites were detected after oral administration in mice to understand the absorption and metabolism of phytochemicals in vivo.
The results showed that the established HPLC method for fingerprint evaluation of PMT has good precision, repeatability, and stability. Additionally, a total of 103 phytochemicals were identified in PMT, including mainly flavonoids and terpenoids. Then, 37 prototype components and 20 derived metabolites in vivo were detected.
In this study, we constructed a fingerprint method, which has good stability, precision, and repeatability, and the fingerprint of PMT was established. Then, the chemical profile of PMT in vitro and in vivo was determined. The results showed that flavonoids and terpenoids were the main phytochemicals in PMT, and methylation, sulfonation, and dihydroxylation were the main metabolic pathway in vivo.
The present study provides the phytochemical basis for subsequent study of pharmacological activity.
豆腐柴是一种传统的药食两用植物，常用于治疗止血、风湿和痢疾。然而，目前对豆腐柴及其体内代谢产物的化学特征仍缺乏清晰的认识。
建立一种快速高效的分析方法，用于鉴定豆腐柴中的植物化学物质及其体内代谢产物。
首先，采用高效液相色谱法建立豆腐柴指纹图谱并进行方法学验证。然后，使用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）鉴定豆腐柴叶的植物化学成分。最后，对小鼠口服给药后检测原型成分和相关代谢产物，以了解植物化学物质在体内的吸收和代谢情况。
结果表明，所建立的用于豆腐柴指纹图谱评价的高效液相色谱法具有良好的精密度、重复性和稳定性。此外，在豆腐柴中总共鉴定出103种植物化学物质，主要包括黄酮类和萜类化合物。随后，检测到37种原型成分和20种体内衍生代谢产物。
本研究构建了一种稳定性、精密度和重复性良好的指纹图谱方法，并建立了豆腐柴指纹图谱。然后，确定了豆腐柴在体外和体内的化学特征。结果表明，黄酮类和萜类化合物是豆腐柴中的主要植物化学物质，甲基化、磺化和二羟基化是体内主要的代谢途径。
本研究为后续药理活性研究提供了植物化学基础。
