Center for Cellular Nanoanalytics (CellNanOs) and Department of Biology and Chemistry, Universität Osnabrück, Barbarastraße 7, 49069 Osnabrück, Germany.
Org Lett. 2024 Oct 25;26(42):9126-9131. doi: 10.1021/acs.orglett.4c03469. Epub 2024 Oct 14.
The -methyl-4-thiophenylpyridinium cation (ThioPy) is a high affinity ( ca. 5 nM), fast-exchanging fluorescent probe for cucurbit[7]uril (CB7). The CB7/ThioPy complex shows a unique fluorescence turn-ON response upon displacement by an analyte in sensing application. This enabled the development of a real-time fluorescence assay with the MRFA peptide for the protease thermolysin, which is also suitable for the cancer biomarker cathepsin B. Moreover, liposome encapsulation of CB7/ThioPy in large unilamellar vesicles (LUVs) provided mechanistic insight into intravesicular dye displacement reactions.
-甲基-4-噻吩基吡啶翁阳离子（ThioPy）是葫芦[7]脲（CB7）的高亲和力（约 5 nM）、快速交换荧光探针。在传感应用中，当被分析物置换时，CB7/ThioPy 配合物表现出独特的荧光开启响应。这使得基于 MRFA 肽的实时荧光测定法得以发展，用于蛋白酶胰凝乳蛋白酶，也适用于癌症生物标志物组织蛋白酶 B。此外，将 CB7/ThioPy 包封在大单层囊泡（LUVs）中的脂质体提供了对囊内染料置换反应的机制见解。
