Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China.
Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China.
Int J Biol Macromol. 2024 Nov;281(Pt 4):136521. doi: 10.1016/j.ijbiomac.2024.136521. Epub 2024 Oct 12.
In this study, A polysaccharide WAAP-2 (121 kDa) with a triple-helical structure was isolated and purified from Agaricus bisporus for the first time. The physicochemical properties, structural characteristics and anti-colon cancer activity were preliminarily investigated. The primary structure indicated that WAAP-2 was composed of mannose, glucose and galactose and determined the position of the linkage between monosaccharide residues. The advanced structure revealed that WAAP-2 has a triple helix and tangled chain conformation. In the anti-colon cancer activity investigation, WAAP-2 exerted an apoptosis-inducing effect by causing HT-29 cell cycle arrest in S phase. WAAP-2 promoted HT-29 cell apoptosis by up-regulating the expression of Caspase-3 and Bax proteins while down-regulating the expression of Bcl-2 protein. Besides, WAAP-2 could inhibit the migration and invasion of colorectal cancer cells by inducing E-cadherin expression and inhibiting Vimentin expression to affect epithelial mesenchymal transition. This paper is of importance for the application of WAAP-2, a triple-helical structural polysaccharide from Agaricus bisporus, to low-toxicity anti-colon cancer drugs.
本研究首次从双孢蘑菇中分离纯化出具有三重螺旋结构的多糖 WAAP-2（121 kDa）。对其理化性质、结构特征和抗结肠癌活性进行了初步研究。初级结构表明 WAAP-2 由甘露糖、葡萄糖和半乳糖组成，并确定了单糖残基之间键的位置。高级结构表明 WAAP-2 具有三重螺旋和缠结链构象。在抗结肠癌活性研究中，WAAP-2 通过使 HT-29 细胞周期停滞在 S 期，发挥诱导细胞凋亡的作用。WAAP-2 通过上调 Caspase-3 和 Bax 蛋白的表达，下调 Bcl-2 蛋白的表达，促进 HT-29 细胞凋亡。此外，WAAP-2 通过诱导 E-钙黏蛋白表达和抑制波形蛋白表达来抑制结肠癌细胞的迁移和侵袭，从而影响上皮间质转化。本文对于将双孢蘑菇中的三重螺旋结构多糖 WAAP-2 应用于低毒性抗结肠癌药物具有重要意义。
