Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, Changchun 130000, China.
Medical Ethics Committee, The Second Hospital of Jilin University, Changchun 130000, China.
Carbohydr Polym. 2017 Jul 1;167:90-96. doi: 10.1016/j.carbpol.2017.03.022. Epub 2017 Mar 9.
A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins. The molecular weight of SPS2p showed only one molecular weight distribution (2.6×10Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59. The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells. These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
从半枝莲全草中分离得到一种水溶性多糖 SPS2p，SPS2p 含有 53.6%碳水化合物、38.5%糖醛酸和 8.2%蛋白质。SPS2p 的分子量仅表现出一个分子量分布（2.6×10 Da），SPS2p 的单糖组成显示存在阿拉伯糖、甘露糖、葡萄糖和半乳糖，比例为 1.31:1.00:3.59:1.59。结果表明，SPS2p 能提高增殖抑制率；SPS2p 还能提高细胞凋亡率、凋亡指数以及 Bax 和 Bak 的水平，降低 Bcl-2 和 FN 的水平；SPS2p 能上调 HT29 细胞中 E-cadherin mRNA 的表达，下调 N-cadherin 和波形蛋白 mRNA 的表达，以及 p-AKT/AKT 的比值。这些结果表明，SPS2p 能抑制 HT29 细胞的增殖和 EMT，促进其凋亡，这可能与 SPS2p 抑制 PI3K/AKT 通路有关。
