Stevenson A P, Martin J C, Stewart C C
Cancer Res. 1986 Jan;46(1):99-105.
Cytostasis and cytotoxicity as well as survival and growth of EMT6 cells following exposure to tumoricidal macrophages have been examined using multiparameter flow cytometry. Macrophages were resolved from tumor cells, and the number of surviving EMT6 cells at different interaction times was determined by adding, to each cell suspension, fluorescent latex particles the concentration of which was known and counting them along with the cells. The percentages of EMT6 cells in S phase were determined by analysis of DNA histograms and compared to the percentages as determined by autoradiography. The progression of cells through the cell cycle was also examined during exposure to macrophages by dual parameter analysis of DNA content and bromodeoxyuridine incorporated into DNA prior to exposure to macrophages. The results showed that, by 4 h of interaction with tumoricidal macrophages, EMT6 cells stopped progressing; tumor cell progression was inhibited in all phases of the cell cycle. By 24 h, there was an absolute decrease in survival. By 48 h, however, surviving tumor cells which escaped the tumoricidal activity of macrophages exhibited a population doubling rate similar to control cells.
利用多参数流式细胞术检测了EMT6细胞在暴露于杀瘤巨噬细胞后的细胞生长抑制、细胞毒性以及存活和生长情况。将巨噬细胞与肿瘤细胞分离，通过向每个细胞悬液中加入已知浓度的荧光乳胶颗粒，并与细胞一起计数，来确定在不同相互作用时间下存活的EMT6细胞数量。通过分析DNA直方图确定S期EMT6细胞的百分比，并与放射自显影法测定的百分比进行比较。在暴露于巨噬细胞期间，还通过对DNA含量和暴露于巨噬细胞之前掺入DNA的溴脱氧尿苷进行双参数分析，来检测细胞在细胞周期中的进展。结果表明，与杀瘤巨噬细胞相互作用4小时后，EMT6细胞停止进展；肿瘤细胞在细胞周期的所有阶段的进展均受到抑制。到24小时时，存活率出现绝对下降。然而，到48小时时，逃脱巨噬细胞杀瘤活性的存活肿瘤细胞表现出与对照细胞相似的群体倍增率。
