Ackermann M F, Lamm K R, Wiegand G W, Luster M I
Immunotoxicology Group, National Institute of Environmental Health Sciences/National Toxicology Program, Triangle Park, North Carolina 27709.
Cancer Res. 1989 Feb 1;49(3):528-32.
The cytostatic and cytolytic activities of activated polymorphonuclear neutrophils (PMNs) against YAC-1 lymphoma target cells were examined using multiparameter flow cytometric analysis. PMNs were resolved from tumor cells by 90 degrees light scatter. The number of surviving tumor cells was determined by adding a known concentration of fluorescent latex particles to the fixed cell suspension immediately prior to analysis and counting the particles simultaneously with the cells. Cell cycle progression of the YAC-1 target was studied by dual parameter analysis of DNA content and bromodeoxyuridine incorporation into tumor cell DNA either prior to or following addition of PMNs. The results indicate that activated PMNs effectively kill tumor cells within the first 24 h of coculture. However, between 24 and 48 h, tumor cells which escape destruction resume growth and eventually reach a growth rate greater than control cells.
使用多参数流式细胞术分析，检测活化的多形核中性粒细胞（PMN）对YAC-1淋巴瘤靶细胞的细胞抑制和细胞溶解活性。通过90度光散射将PMN与肿瘤细胞区分开。在分析前立即向固定的细胞悬液中加入已知浓度的荧光乳胶颗粒，并与细胞同时计数，以此确定存活肿瘤细胞的数量。通过在加入PMN之前或之后对DNA含量和溴脱氧尿苷掺入肿瘤细胞DNA进行双参数分析，研究YAC-1靶细胞的细胞周期进程。结果表明，活化的PMN在共培养的前24小时内有效杀死肿瘤细胞。然而，在24至48小时之间，逃脱破坏的肿瘤细胞恢复生长，最终达到比对照细胞更高的生长速率。
