Long Jiao, Gao Yan, Sheng Kangjia, Bao Tao, Wang Sicen
School of Pharmacy, Xi'an Jiaotong University Health Science Center, Xi'an, China.
Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis, Xi'an, China.
Electrophoresis. 2025 May;46(9-10):562-570. doi: 10.1002/elps.202400096. Epub 2024 Oct 14.
In this study, an efficient and practical method was established to enrich and detect three trace quinolones in fish tissue. Low-cost and environment-friendly cotton threads were coated with PCN-224 using a simple one-pot hydrothermal method. The PCN-224-modified cotton threads (PCN-224@CTs) were characterized to ensure that the crystal was successfully fabricated on the surface of cotton threads. The prepared PCN-224@CTs were wrapped around steel needles to form suspended bars. Key factors of extraction and desorption were optimized, and analytical performance was tested. Suspended bar sorptive extraction coupled with ultra-performance liquid chromatography (UPLC) with a photodiode array detector was used to analyze the quinolones in fish tissue. The detection limits of quinolones were 0.26-1.15 ng/mL, and the recoveries were achieved in the range of 81.31%-115.81%, indicating a simple method with high sensitivity and satisfactory practicability of enriching, separating, and detecting trace quinolones was successfully established.
在本研究中，建立了一种高效实用的方法来富集和检测鱼类组织中的三种痕量喹诺酮类药物。采用简单的一锅水热法将低成本且环保的棉线用PCN-224进行包覆。对PCN-224修饰的棉线（PCN-224@CTs）进行表征，以确保晶体成功制备在棉线表面。将制备好的PCN-224@CTs缠绕在钢针上形成悬挂棒。对萃取和解吸的关键因素进行了优化，并测试了分析性能。采用悬挂棒吸附萃取结合配备光电二极管阵列检测器的超高效液相色谱（UPLC）对鱼类组织中的喹诺酮类药物进行分析。喹诺酮类药物的检测限为0.26 - 1.15 ng/mL，回收率在81.31% - 115.81%范围内，表明成功建立了一种简单、具有高灵敏度且富集、分离和检测痕量喹诺酮类药物实用性良好的方法。
