College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, P. R. China.
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, P. R. China.
J Sep Sci. 2023 Jul;46(13):e2200833. doi: 10.1002/jssc.202200833. Epub 2023 May 6.
A novel analytical method based on stir-bar sorptive extraction was proposed for the determination of three trace quinolones in fish and shrimp samples. UiO-66-(OH) , a hydroxyl-functionalized zirconium metal-organic framework, has been coated on frosted glass rods by an in situ growth method. The product, UiO-66-(OH) modified frosted glass rods, has been characterized and key parameters have been optimized in combination with ultra-high-performance liquid chromatography. The detection limits of enoxacin, norfloxacin, and ciprofloxacin were 0.48-0.8 ng ml , and the detection concentrations were in the range of 10-300 ng ml , showing a good linear relationship. This method was used for the determination of three quinolones in aquatic organisms, and the recoveries in spiked fish and shrimp muscle tissue samples were 74.8%-105.4% and 82.5%-115.8%, respectively. The relative standard deviations were less than 6.9%. The established method combined stir-bar sorptive extraction based on UiO-66-(OH) modified frosted glass rods with ultra-high-performance liquid chromatography, has good application prospects for the detection of quinolone residues in fish and shrimp muscle samples.
基于搅拌棒吸附萃取的新型分析方法被提出，用于测定鱼和虾样品中的三种痕量喹诺酮类药物。UiO-66-(OH)，一种羟基功能化的锆金属有机骨架，通过原位生长法涂覆在磨砂玻璃棒上。产物 UiO-66-(OH)修饰的磨砂玻璃棒进行了表征，并与超高效液相色谱法相结合优化了关键参数。恩诺沙星、诺氟沙星和环丙沙星的检测限为 0.48-0.8ngml，检测浓度范围为 10-300ngml，呈现出良好的线性关系。该方法用于测定水生生物中的三种喹诺酮类药物，在鱼和虾肌肉组织样品中的加标回收率分别为 74.8%-105.4%和 82.5%-115.8%。相对标准偏差小于 6.9%。建立的方法将基于 UiO-66-(OH)修饰的磨砂玻璃棒的搅拌棒吸附萃取与超高效液相色谱相结合，在检测鱼和虾肌肉样品中的喹诺酮类药物残留方面具有良好的应用前景。
