Purnianto Adityas, Mawal Celeste, Kulkarni Mitali M, Su Huaqi, Koukoulis Tiana F, Wongsodirdjo Patricia, Hung Ya Hui, Ayton Scott, Bush Ashley I, Barnham Kevin J, Vella Laura J
The Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria Australia.
Department of Surgery, The Royal Melbourne Hospital, Australia The University of Melbourne Parkville Victoria Australia.
J Extracell Biol. 2024 Oct 14;3(10):e70012. doi: 10.1002/jex2.70012. eCollection 2024 Oct.
Cells have developed a highly regulated system for the uptake, transport, utilization, storage, and export of metals, ensuring the maintenance of cellular homeostasis. Small extracellular vesicles (sEVs) function as a mechanism through which a cell can export its cargo and transfer it to recipient cells. However, in contrast to the other molecular cargo associated with sEVs, the metal content of sEVs is not well characterized. To address this gap in knowledge, we measured the levels of nine essential metals (copper, iron, zinc, manganese, magnesium, potassium, calcium, chromium, cobalt) and six non-essential metals (nickel, rubidium, titanium, aluminium, lithium, lead) in sEVs originating from multiple in vitro and sources. Our findings reveal that, beyond containing redox-active essential metals and those involved in redox reactions, sEVs also exhibit the capability to export and transfer non-physiological, potentially toxic metals.
细胞已经发展出一套高度调节的系统，用于金属的摄取、运输、利用、储存和输出，以确保维持细胞内稳态。小细胞外囊泡（sEVs）作为一种机制，细胞可通过它输出其货物并将其转移到受体细胞。然而，与与sEVs相关的其他分子货物相比，sEVs的金属含量尚未得到很好的表征。为了填补这一知识空白，我们测量了源自多种体外来源的sEVs中九种必需金属（铜、铁、锌、锰、镁、钾、钙、铬、钴）和六种非必需金属（镍、铷、钛、铝、锂、铅）的水平。我们的研究结果表明，除了含有氧化还原活性必需金属和参与氧化还原反应的金属外，sEVs还具有输出和转移非生理性、潜在有毒金属的能力。
