Hunan Provincial Key Laboratory of Traditional Chinese Medicine (TCM) Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, China.
Front Immunol. 2024 Sep 30;15:1463767. doi: 10.3389/fimmu.2024.1463767. eCollection 2024.
Linarine is a natural chemical component widely found in Buddleja officinalis Maxim., Chrysanthemum indicum L., Mentha canadensis L., and other medicinal plants. Modern pharmacological studies have shown that linarine with good anti-inflammatory and antioxidant activities can inhibit the proliferation and induce apoptosis of many kinds of tumor cells. Moreover, linarine showed protective effect on the liver, kidneys, and other organs.
Inflammation model of human corneal epithelial cell (HCEC) was constructed using NaCl induction, and cytotoxicity was detected by the CCK8 assay. The levels of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β) were measured using Enzyme-linked immunoassay (ELISA). Chronic painful stimulation (tail clamping) in combination with Benzalkonium Chloride Solution drops in a desiccator established a mouse model of dry eye disease (DED). The following parameters were recorded: body mass, anal temperature, tear secretion, tear film rupture time, and corneal fluorescein staining. The levels of inflammatory factors mitogen activated protein kinase (MAPK), nuclear factor kappa-B (NF-kB), c-Jun N-terminal kinase (JNK), IL-1β, Interleukin 18(IL-18), A2A, A3, P2X4, P2X7, P2Y1 were measured by using immunofluorescence (IF) staining.
Linarine can inhibit the secreation of TNF-α, and IL-1β in HCECs. Linarine prolonged tear film rupture time, promoted tear secretion, repaired corneal damage, and reduced the levels of inflammatory factors of MAPK, NF-kB, JNK, IL-1β, IL-18, and modulated the levels of the purinergic receptor.
Linarine is effective in treating dry eye in mice by inhibiting purinergic receptors-mediated inflammatory response.
梓醇是一种天然化学物质，广泛存在于醉鱼草、野菊花、薄荷等药用植物中。现代药理学研究表明，具有良好抗炎和抗氧化活性的梓醇可以抑制多种肿瘤细胞的增殖并诱导其凋亡，对肝脏、肾脏等器官具有保护作用。
采用 NaCl 诱导人角膜上皮细胞（HCEC）构建炎症模型，用 CCK8 法检测细胞毒性。酶联免疫吸附法（ELISA）检测肿瘤坏死因子-α（TNF-α）和白细胞介素 1β（IL-1β）等炎症因子的水平。在干燥器中用尾夹夹尾结合苯扎氯铵溶液滴注的方法建立小鼠干眼症（DED）模型。记录体重、肛温、泪液分泌、泪膜破裂时间和角膜荧光素染色。免疫荧光（IF）染色法检测丝裂原活化蛋白激酶（MAPK）、核因子 κB（NF-κB）、c-Jun N-末端激酶（JNK）、IL-1β、白细胞介素 18（IL-18）、A2A、A3、P2X4、P2X7、P2Y1 等炎症因子的水平。
梓醇可抑制 HCEC 中 TNF-α和 IL-1β的分泌。梓醇延长泪膜破裂时间，促进泪液分泌，修复角膜损伤，降低 MAPK、NF-κB、JNK、IL-1β、IL-18 等炎症因子水平，并调节嘌呤能受体水平。
梓醇通过抑制嘌呤能受体介导的炎症反应，对小鼠干眼症有治疗作用。
