Mahdavi Farzad, Mohammadi Mohammad Reza, Karimi Kambiz, Shamsi Laya, Maleki Farajolah, Asghari Ali, Shahabi Saeed, Motazedian Mohammad Hossein, Nourmohammadi Hassan
Department of Medical Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Faculty of Medical Sciences, Department of Bacteriology, Tarbiat Modares University, Tehran, Iran.
Foodborne Pathog Dis. 2025 Jul;22(7):498-504. doi: 10.1089/fpd.2024.0100. Epub 2024 Oct 15.
This study aimed to investigate the clinical and molecular characteristics of () infection and identify potential risk factors in children and teenagers with malignancies in Shiraz, southwestern Iran. A total of 200 fresh fecal samples were collected from children and adolescents suffering from 32 different cancer types at Amir, Nemazee, and Saadi hospitals affiliated with Shiraz University of Medical Sciences between October 2021 and May 2022. Direct microscopy using saline and iodine wet mount was conducted, and all fecal samples were rechecked by -PCR. Subsequently, a specific fragment of the gene was amplified on all samples for prevalence, sequencing, and assemblage identification. Our study found a 4% (8/200) prevalence of using microscopy and PCR. The molecular findings were consistent with the microscopic results. All eight positive samples with gene were also detected as positive with gene and were correctly sequenced. Among the examined cancer patients, two assemblages were identified: A [sub-assemblage AI (2/8, 25%) and sub-assemblage AII (3/8, 37.5%)] and B [sub-assemblage BIV (3/8, 37.5%)]. Notably, patients were more vulnerable to infection after receiving at least 8 treatment episodes ( < 0.05) and displaying gastrointestinal symptoms ( > 0.05). The demographic characteristics of cancer patients with infection and the statistical conclusions were separately detailed. The small sample size and low prevalence rate in this study hindered precise epidemiological conclusions. Nonetheless, the results suggest that infection among cancer patients in Shiraz city originates from humans, without any specific animal groups (C-H) involved.
本研究旨在调查（）感染的临床和分子特征，并确定伊朗西南部设拉子市患有恶性肿瘤的儿童和青少年的潜在危险因素。2021年10月至2022年5月期间，从设拉子医科大学附属的阿米尔、内马齐和萨阿迪医院的患有32种不同癌症类型的儿童和青少年中总共收集了200份新鲜粪便样本。采用生理盐水和碘液湿片法进行直接显微镜检查，所有粪便样本均通过 -PCR重新检查。随后，对所有样本扩增该基因的特定片段以进行流行率、测序和组合鉴定。我们的研究发现，使用显微镜检查和PCR检测到（）的流行率为4%（8/200）。分子检测结果与显微镜检查结果一致。所有8份该基因阳性样本经该基因检测也为阳性，并成功测序。在所检查的癌症患者中，鉴定出两种组合：A [亚组合AI（2/8，25%）和亚组合AII（3/8，37.5%）]和B [亚组合BIV（3/8，37.5%）]。值得注意的是，患者在接受至少8次治疗后更容易感染（<0.05），并且出现胃肠道症状（>0.05）。分别详细列出了感染（）的癌症患者的人口统计学特征和统计结论。本研究样本量小且患病率低，阻碍了得出精确的流行病学结论。尽管如此，结果表明设拉子市癌症患者中的（）感染源自人类，没有涉及任何特定动物群体（C-H）。
