Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Comp Immunol Microbiol Infect Dis. 2022 Jul;86:101819. doi: 10.1016/j.cimid.2022.101819. Epub 2022 May 11.
Livestock are commonly affected by gastrointestinal protozoan parasites, including Giardia duodenalis. In small ruminants, G. duodenalis infection may decrease carcass weight and dressing percentage. Current study was done to determine the prevalence, assemblage distribution, and zoonotic significance of Giardia infection in sheep and goats in Shiraz, southwestern Iran. In total, 200 fecal samples were randomly collected from 100 sheep and 100 goats in 10 farms in Shiraz, southwest of Iran (June 2021-February 2022) and directly examined for G. duodenalis cyst by saline/iodine wet mount examination using a light microscope (400 ×). Positive samples were further genotyped using a nested-PCR and sequencing methods. A mean prevalence of 5.5% (11/200) was estimated for G. duodenalis infection among small ruminants, with 7% and 4% for sheep and goats, respectively. Next, 9 out of 11 positive samples were amplified and only 5 were successfully sequenced at the tpi locus. Our results showed that 80% (4/5) isolates belonged to assemblage E, while only 20% (1/5) were associated with the assemblage A (subtype AI). Of note, 2 E assemblages were isolated from goats and 2 E assemblages plus 1 assemblage A were isolated from sheep. The major finding in the present study was the isolation of assemblage A from sheep in Shiraz, highlighting the zoonotic transmission of Giardia infection in the study area. In general, the information mentioned in the present study is very limited and more extensive studies in this field are needed to achieve more conclusive results.
家畜通常会受到胃肠道原生动物寄生虫的影响，包括十二指肠贾第虫。在小反刍动物中，十二指肠贾第虫感染可能会降低胴体重和屠宰率。本研究旨在确定伊朗西南部设拉子地区绵羊和山羊中贾第虫感染的流行率、种群分布和人畜共患意义。2021 年 6 月至 2022 年 2 月期间，从设拉子的 10 个农场中随机采集了 200 份来自 100 只绵羊和 100 只山羊的粪便样本，并使用生理盐水/碘湿载片镜检法（400×）直接检查十二指肠贾第虫包囊。对阳性样本进一步采用巢式 PCR 和测序方法进行基因分型。在小反刍动物中，十二指肠贾第虫感染的总流行率为 5.5%（11/200），绵羊和山羊的流行率分别为 7%和 4%。随后，在 11 个阳性样本中扩增出 9 个，只有 5 个在 tpi 基因座成功测序。结果显示，80%（4/5）的分离株属于 E 种群，而只有 20%（1/5）与 A 种群（AI 亚型）有关。值得注意的是，从山羊中分离出 2 个 E 种群，从绵羊中分离出 2 个 E 种群和 1 个 A 种群。本研究的主要发现是在设拉子从绵羊中分离出 A 种群，强调了该研究地区贾第虫感染的人畜共患传播。总的来说，本研究中提到的信息非常有限，需要在这一领域进行更广泛的研究，以得出更具结论性的结果。
